Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM009587-33A2
Application #
2168482
Study Section
Biochemistry Study Section (BIO)
Project Start
1975-01-01
Project End
1999-06-30
Budget Start
1996-07-01
Budget End
1997-06-30
Support Year
33
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Iowa State University
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
City
Ames
State
IA
Country
United States
Zip Code
50011
Huang, C Y; Yuan, C J; Luo, S et al. (1994) Mutational analyses of the metal ion and substrate binding sites of phosphorylase kinase gamma subunit. Biochemistry 33:5877-83
Luo, S; Huang, C Y; McClelland, J F et al. (1994) A study of protein secondary structure by Fourier transform infrared/photoacoustic spectroscopy and its application for recombinant proteins. Anal Biochem 216:67-76
Yuan, C J; Huang, C Y; Graves, D J (1994) Oxidation and site-directed mutagenesis of the sulfhydryl groups of a truncated gamma catalytic subunit of phosphorylase kinase. Functional and structural effects. J Biol Chem 269:24367-73
Martin, B L; Graves, D J (1994) Isotope effects on the mechanism of calcineurin catalysis: kinetic solvent isotope and isotope exchange studies. Biochim Biophys Acta 1206:136-42
Graves, D J; Luo, S (1994) Use of photoacoustic Fourier-transform infrared spectroscopy to study phosphates in proteins. Biochem Biophys Res Commun 205:618-24
Yuan, C J; Huang, C Y; Graves, D J (1993) Phosphorylase kinase, a metal ion-dependent dual specificity kinase. J Biol Chem 268:17683-6
Huang, C Y; Yuan, C J; Livanova, N B et al. (1993) Expression, purification, characterization, and deletion mutations of phosphorylase kinase gamma subunit: identification of an inhibitory domain in the gamma subunit. Mol Cell Biochem 127-128:7-18
Martin, B L; Graves, D J (1993) Hydrolysis of trifluoroethyl phosphate as evidence that the serine and tyrosine phosphatase activities of calcineurin share the same specificity determinant. Biochem Biophys Res Commun 194:150-6
Wang, H; Graves, D J (1991) Calcineurin-catalyzed reaction with phosphite and phosphate esters of tyrosine. Biochemistry 30:3019-24
Takrama, J F; Graves, D J (1991) Solution conformations of the N-terminal CNBr fragment of glycogen phosphorylase and its interaction with calmodulin. Biochim Biophys Acta 1077:371-8

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