Abstract

One goal of this project is the general improvement and expansion of the methodology of field-flow fractionation (FFF). Efforts will be made to opotimize speed, resolution, range and accuracy through improved channels, seal design, new FFF systems and improved procedures. Collaborative projects will be undertaken to extend the applicability of FFF into diverse biomedical fields. These projects will involve the separation and characterization of viral aggregates, subcellular particles, bile micelles, synthetic blood, viral particles, acidic peptides, polynucleotides, and the protein structures in cataract impaired lenses. Finally, theoretical studies will be continued to clarify the relationship between diverse separation methods.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM010851-29
Application #
3268160
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1979-02-01
Project End
1987-01-31
Budget Start
1986-02-01
Budget End
1987-01-31
Support Year
29
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Arts and Sciences
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Benincasa, M A; Caldwell, K D (2001) Flow field-flow fractionation of poly(ethylene oxide): effect of carrier ionic strength and composition. J Chromatogr A 925:159-69
Tong, X; Caldwell, K D (1999) Computer simulation of particle separation based on non-equilibrium swelling. J Chromatogr A 831:51-62
Vauthier, J C; Williams, P S (1998) Numerical simulation of band-broadening during hydrodynamic relaxation in frit-inlet field-flow fractionation channels. J Chromatogr A 805:149-60
Jensen, K D; Williams, S K; Giddings, J C (1996) High-speed particle separation and steric inversion in thin flow field-flow fractionation channels. J Chromatogr A 746:137-45
Giddings, J C (1995) Sample dimensionality: a predictor of order-disorder in component peak distribution in multidimensional separation. J Chromatogr A 703:3-15
Giddings, J C; Xu, Y; Myers, M N (1994) Enhancement of performance in sedimentation field-flow fractionation by temperature elevation. Anal Chem 66:3047-53
Williams, P S; Giddings, J C (1994) Theory of field-programmed field-flow fractionation with corrections for steric effects. Anal Chem 66:4215-28
Giddings, J C (1993) Field-flow fractionation: analysis of macromolecular, colloidal, and particulate materials. Science 260:1456-65
Barman, B N; Ashwood, E R; Giddings, J C (1993) Separation and size distribution of red blood cells of diverse size, shape, and origin by flow/hyperlayer field-flow fractionation. Anal Biochem 212:35-42
Moon, M H; Giddings, J C (1993) Size distribution of liposomes by flow field-flow fractionation. J Pharm Biomed Anal 11:911-20

Showing the most recent 10 out of 36 publications