The long term objective of this research proposal is to develop and expand field-flow fractionation (FFF) technology in such a way that its intrinsic advantages can be readily realized and applied to complex biochemical materials. Another objective is to push back the frontiers of separation methodology. The above objectives are to be met by pursuing work in four categories: 1. General Optimization of FFF. Here we weill seek improvements in instrumentation, channel design, and procedures in order to make FFF more accessible and more useful to the broad scientific community. As part of this study, we will examine the optimization of programmed FFF. 2. Biochemical Applications. We will engage in several projects of direct biochemical relevance, working mainly with collaborators from the biomedical community. 3. Two-Dimensional FFF. We will undertake a major long-term initiative to combine the high resolving power and versatility of two-dimensional methodology with the advantage displayed by FFF in the separation of macromolecular and particulate components. In this way we hope to evolve new and powerful separation techniques. 4. Developments in Separation Science. Here we will continue our long-standing efforts to broadly advance the theoretical understanding of basic separation processes. In addition, we will seek to develop new separation approaches and techniques.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM010851-32
Application #
3268162
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1979-02-01
Project End
1992-01-31
Budget Start
1989-02-01
Budget End
1990-01-31
Support Year
32
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Utah
Department
Type
Schools of Arts and Sciences
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Benincasa, M A; Caldwell, K D (2001) Flow field-flow fractionation of poly(ethylene oxide): effect of carrier ionic strength and composition. J Chromatogr A 925:159-69
Tong, X; Caldwell, K D (1999) Computer simulation of particle separation based on non-equilibrium swelling. J Chromatogr A 831:51-62
Vauthier, J C; Williams, P S (1998) Numerical simulation of band-broadening during hydrodynamic relaxation in frit-inlet field-flow fractionation channels. J Chromatogr A 805:149-60
Jensen, K D; Williams, S K; Giddings, J C (1996) High-speed particle separation and steric inversion in thin flow field-flow fractionation channels. J Chromatogr A 746:137-45
Giddings, J C (1995) Sample dimensionality: a predictor of order-disorder in component peak distribution in multidimensional separation. J Chromatogr A 703:3-15
Giddings, J C; Xu, Y; Myers, M N (1994) Enhancement of performance in sedimentation field-flow fractionation by temperature elevation. Anal Chem 66:3047-53
Williams, P S; Giddings, J C (1994) Theory of field-programmed field-flow fractionation with corrections for steric effects. Anal Chem 66:4215-28
Giddings, J C (1993) Field-flow fractionation: analysis of macromolecular, colloidal, and particulate materials. Science 260:1456-65
Barman, B N; Ashwood, E R; Giddings, J C (1993) Separation and size distribution of red blood cells of diverse size, shape, and origin by flow/hyperlayer field-flow fractionation. Anal Biochem 212:35-42
Moon, M H; Giddings, J C (1993) Size distribution of liposomes by flow field-flow fractionation. J Pharm Biomed Anal 11:911-20

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