Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM011632-33
Application #
2168549
Study Section
Biophysical Chemistry Study Section (BBCB)
Project Start
1977-09-01
Project End
1997-08-31
Budget Start
1995-09-01
Budget End
1997-08-31
Support Year
33
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Brown, M P; Shaikh, N; Brenowitz, M et al. (1994) The allosteric interaction between D-galactose and the Escherichia coli galactose repressor protein. J Biol Chem 269:12600-5
Wu, P; Li, Y K; Talalay, P et al. (1994) Characterization of the three tyrosine residues of delta 5-3-ketosteroid isomerase by time-resolved fluorescence and circular dichroism. Biochemistry 33:7415-22
Wu, P; Brand, L (1994) Resonance energy transfer: methods and applications. Anal Biochem 218:1-13
Chauvin, F; Brand, L; Roseman, S (1994) Sugar transport by the bacterial phosphotransferase system. Characterization of the Escherichia coli enzyme I monomer/dimer transition kinetics by fluorescence anisotropy. J Biol Chem 269:20270-4
Wu, P; Brand, L (1994) Conformational flexibility in a staphylococcal nuclease mutant K45C from time-resolved resonance energy transfer measurements. Biochemistry 33:10457-62
Chauvin, F; Brand, L; Roseman, S (1994) Sugar transport by the bacterial phosphotransferase system. Characterization of the Escherichia coli enzyme I monomer/dimer equilibrium by fluorescence anisotropy. J Biol Chem 269:20263-9
Hirshfield, K M; Toptygin, D; Packard, B S et al. (1993) Dynamic fluorescence measurements of two-state systems: applications to calcium-chelating probes. Anal Biochem 209:209-18
Wu, P G; James, E; Brand, L (1993) Compact thermally-denatured state of a staphylococcal nuclease mutant from resonance energy transfer measurements. Biophys Chem 48:123-33
Toptygin, D; Brand, L (1993) Fluorescence decay of DPH in lipid membranes: influence of the external refractive index. Biophys Chem 48:205-20
Rice, K G; Wu, P; Brand, L et al. (1993) Modification of oligosaccharide antenna flexibility induced by exoglycosidase trimming. Biochemistry 32:7264-70

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