Abstract

The object is to elucidate the mechanism of enzyme catalyzed reactions and related phenomena such as ligand binding by hemoglobin, and protein-protein interactions, by making observations on the chemical and physical properties of appropriate systems, often of a kinetic character. The observations will be compared with suitable model systems and correlated with structural and chemical information available to build up a descriptive scheme best approximating the experiment.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM014276-23
Application #
3268613
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1979-06-01
Project End
1989-05-31
Budget Start
1987-06-01
Budget End
1988-05-31
Support Year
23
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Cornell University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850

  Publications

Carlson, M L; Regan, R; Elber, R et al. (1994) Nitric oxide recombination to double mutants of myoglobin: role of ligand diffusion in a fluctuating heme pocket. Biochemistry 33:10597-606
Chiancone, E; Elber, R; Royer Jr, W E et al. (1993) Ligand binding and conformation change in the dimeric hemoglobin of the clam Scapharca inaequivalvis. J Biol Chem 268:5711-8
Gibson, Q H; Regan, R; Olson, J S et al. (1993) Kinetics of ligand binding to Pseudoterranova decipiens and Ascaris suum hemoglobins and to Leu-29-->Tyr sperm whale myoglobin mutant. J Biol Chem 268:16993-8
Bellelli, A; Lendaro, E; Ippoliti, R et al. (1993) Ligand binding and slow structural changes in chlorocruorin from Spirographis spallanzanii. Biochemistry 32:7635-43
Blackmore, R S; Gibson, Q H; Greenwood, C (1992) The relation of ligand binding to redox state in the hexa-heme nitrite reductase of Wolinella succinogenes. J Biol Chem 267:10950-5
Gibson, Q H; Bellelli, A; Regan, R et al. (1992) Ligand binding by the chlorocruorin from Eudistylia vancouverii. J Biol Chem 267:11977-81
Gibson, Q H; Regan, R; Elber, R et al. (1992) Distal pocket residues affect picosecond ligand recombination in myoglobin. An experimental and molecular dynamics study of position 29 mutants. J Biol Chem 267:22022-34
Blackmore, R S; Greenwood, C; Gibson, Q H (1991) Studies of the primary oxygen intermediate in the reaction of fully reduced cytochrome oxidase. J Biol Chem 266:19245-9
Carver, T E; Olson, J S; Smerdon, S J et al. (1991) Contributions of residue 45(CD3) and heme-6-propionate to the biomolecular and geminate recombination reactions of myoglobin. Biochemistry 30:4697-705
Smerdon, S J; Dodson, G G; Wilkinson, A J et al. (1991) Distal pocket polarity in ligand binding to myoglobin: structural and functional characterization of a threonine68(E11) mutant. Biochemistry 30:6252-60

Showing the most recent 10 out of 26 publications