Abstract

The initiation of sporulation in Bacillus subtilis is genetically controlled by a number of loci. These loci, spoOA, spoOB, etc., are highly pleiotropic, preventing the synthesis of all sporulation related sporulation functions. The major goal of the proposed research is to isolate and characterize the products of the spoO loci and to determine how these products control the initiation of differentiation. This goal is being approached by the molecular cloning of these genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM019416-15
Application #
3269638
Study Section
(MG)
Project Start
1977-05-01
Project End
1987-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
15
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92037

  Publications

Feinauer, Christoph; Szurmant, Hendrik; Weigt, Martin et al. (2016) Inter-Protein Sequence Co-Evolution Predicts Known Physical Interactions in Bacterial Ribosomes and the Trp Operon. PLoS One 11:e0149166
Wu, R; Gu, M; Wilton, R et al. (2013) Insight into the sporulation phosphorelay: crystal structure of the sensor domain of Bacillus subtilis histidine kinase, KinD. Protein Sci 22:564-76
Pokkuluri, P Raj; Dwulit-Smith, Jeff; Duke, Norma E et al. (2013) Analysis of periplasmic sensor domains from Anaeromyxobacter dehalogenans 2CP-C: structure of one sensor domain from a histidine kinase and another from a chemotaxis protein. Microbiologyopen 2:766-77
Perego, Marta (2013) Forty years in the making: understanding the molecular mechanism of peptide regulation in bacterial development. PLoS Biol 11:e1001516
Szurmant, Hendrik; Hoch, James A (2013) Statistical analyses of protein sequence alignments identify structures and mechanisms in signal activation of sensor histidine kinases. Mol Microbiol 87:707-12
Bobay, Benjamin G; Hoch, James A; Cavanagh, John (2012) Dynamics and activation in response regulators: the ?4-?4 loop. Biomol Concepts 3:175-182
Diaz, Alejandra R; Core, Leighton J; Jiang, Min et al. (2012) Bacillus subtilis RapA phosphatase domain interaction with its substrate, phosphorylated Spo0F, and its inhibitor, the PhrA peptide. J Bacteriol 194:1378-88
Dago, Angel E; Schug, Alexander; Procaccini, Andrea et al. (2012) Structural basis of histidine kinase autophosphorylation deduced by integrating genomics, molecular dynamics, and mutagenesis. Proc Natl Acad Sci U S A 109:E1733-42
Procaccini, Andrea; Lunt, Bryan; Szurmant, Hendrik et al. (2011) Dissecting the specificity of protein-protein interaction in bacterial two-component signaling: orphans and crosstalks. PLoS One 6:e19729
Baker, Kris Ann; Perego, Marta (2011) Transcription antitermination by a phosphorylated response regulator and cobalamin-dependent termination at a B?? riboswitch contribute to ethanolamine utilization in Enterococcus faecalis. J Bacteriol 193:2575-86

Showing the most recent 10 out of 29 publications