Abstract

We propose to study development using the bacterium Myxococcus xanthus as a model system. Our specific objectives are: (a) To characterize myxobacterial hemagglutinin (MBHA), a development specific protein which peaks at the time of cellular aggregation. We plan to search for the MBHA receptor in the cell membrane and characterize it. (b) To isolate MBHA and protein S defective mutants of M. xanthus by direct screening of hundreds of developmental mutants in our collection. Mutants which are obtained will be characterized using biochemical, physiological and genetic techniques. (c) To study transcriptional regulation of development. The RNA polymerase from vegetative and developmental extracts will be studied on several templates in order to study developmentally regulated transcription in vitro.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM020509-13
Application #
3270067
Study Section
(MG)
Project Start
1976-06-01
Project End
1987-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Fu, Guo; Bandaria, Jigar N; Le Gall, Anne Valérie et al. (2018) MotAB-like machinery drives the movement of MreB filaments during bacterial gliding motility. Proc Natl Acad Sci U S A 115:2484-2489
Berleman, James E; Zemla, Marcin; Remis, Jonathan P et al. (2016) Exopolysaccharide microchannels direct bacterial motility and organize multicellular behavior. ISME J 10:2620-2632
Nan, Beiyan; Zusman, David R (2016) Novel mechanisms power bacterial gliding motility. Mol Microbiol 101:186-93
Kaimer, Christine; Zusman, David R (2016) Regulation of cell reversal frequency in Myxococcus xanthus requires the balanced activity of CheY-like domains in FrzE and FrzZ. Mol Microbiol 100:379-95
Nan, Beiyan; Bandaria, Jigar N; Guo, Kathy Y et al. (2015) The polarity of myxobacterial gliding is regulated by direct interactions between the gliding motors and the Ras homolog MglA. Proc Natl Acad Sci U S A 112:E186-93
Nan, Beiyan; McBride, Mark J; Chen, Jing et al. (2014) Bacteria that glide with helical tracks. Curr Biol 24:R169-73
Moine, Audrey; Agrebi, Rym; Espinosa, Leon et al. (2014) Functional organization of a multimodular bacterial chemosensory apparatus. PLoS Genet 10:e1004164
Kaimer, Christine; Zusman, David R (2013) Phosphorylation-dependent localization of the response regulator FrzZ signals cell reversals in Myxococcus xanthus. Mol Microbiol 88:740-53
Nan, Beiyan; Bandaria, Jigar N; Moghtaderi, Amirpasha et al. (2013) Flagella stator homologs function as motors for myxobacterial gliding motility by moving in helical trajectories. Proc Natl Acad Sci U S A 110:E1508-13
Kaimer, Christine; Berleman, James E; Zusman, David R (2012) Chemosensory signaling controls motility and subcellular polarity in Myxococcus xanthus. Curr Opin Microbiol 15:751-7

Showing the most recent 10 out of 24 publications