Abstract

The structure of the 5'-terminus of mRNAs coding for conalbumin, lysozyme and ovomucoid in hen oviduct will be determined with respect to nucleotide sequence, methylation pattern, and site(s) of transcriptional initiation on the gene. The binding of cap analogues and capped oligonucleotides to cap-binding protein will be determined. Changes in the fluorescence of 7-methylguanosine in the cap of mRNA will be studied upon interaction with cap-binding protein.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM020818-13
Application #
3270168
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1977-04-01
Project End
1988-01-18
Budget Start
1986-12-01
Budget End
1988-01-18
Support Year
13
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Kentucky
Department
Type
Schools of Medicine
DUNS #
832127323
City
Lexington
State
KY
Country
United States
Zip Code
40506

  Publications

Ziemniak, Marcin; Mugridge, Jeffrey S; Kowalska, Joanna et al. (2016) Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex. RNA 22:518-29
Korneeva, Nadejda L; Song, Anren; Gram, Hermann et al. (2016) Inhibition of Mitogen-activated Protein Kinase (MAPK)-interacting Kinase (MNK) Preferentially Affects Translation of mRNAs Containing Both a 5'-Terminal Cap and Hairpin. J Biol Chem 291:3455-67
Fan, Ruping; Schrott, Lisa M; Snelling, Stephen et al. (2015) Chronic oxycodone induces integrated stress response in rat brain. BMC Neurosci 16:58
Kowalska, Joanna; Wypijewska del Nogal, Anna; Darzynkiewicz, Zbigniew M et al. (2014) Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes. Nucleic Acids Res 42:10245-64
Grudzien-Nogalska, Ewa; Reed, Brent C; Rhoads, Robert E (2014) CPEB1 promotes differentiation and suppresses EMT in mammary epithelial cells. J Cell Sci 127:2326-38
Ziemniak, Marcin; Szabelski, Mariusz; Lukaszewicz, Maciej et al. (2013) Synthesis and evaluation of fluorescent cap analogues for mRNA labelling. RSC Adv 3:
Su, Wei; Slepenkov, Sergey V; Slevin, Michael K et al. (2013) mRNAs containing the histone 3' stem-loop are degraded primarily by decapping mediated by oligouridylation of the 3' end. RNA 19:1-16
Chiluiza, David; Bargo, Sharon; Callahan, Robert et al. (2011) Expression of truncated eukaryotic initiation factor 3e (eIF3e) resulting from integration of mouse mammary tumor virus (MMTV) causes a shift from cap-dependent to cap-independent translation. J Biol Chem 286:31288-96
Senkovich, Olga A; Yin, Jun; Ekshyyan, Viktoriya et al. (2011) Helicobacter pylori AlpA and AlpB bind host laminin and influence gastric inflammation in gerbils. Infect Immun 79:3106-16
Su, Wei; Slepenkov, Sergey; Grudzien-Nogalska, Ewa et al. (2011) Translation, stability, and resistance to decapping of mRNAs containing caps substituted in the triphosphate chain with BH3, Se, and NH. RNA 17:978-88

Showing the most recent 10 out of 30 publications