Abstract

The goal of this research is to obtain metrical information from continuous wave and pulsed EPR spectroscopy of interacting spin systems in biological molecules. EPR transition energies, transition probabilities, and relaxation times will be calculated for spin systems of interest. These calculations will be calibrated with discrete metal complexes containing paramagnetic metals and nitroxyl spin labels. Exchange and dipolar contributions to the spin-spin interaction will be measured. The limits of applicability of various approaches to measuring spin-spin distances will be determined. These results will be applied to the nickel metalloenzyme urease, the copper-containing plastocyanin, and to CaATPase.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM021156-11
Application #
3270287
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1977-07-01
Project End
1989-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
11
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Denver
Department
Type
Schools of Arts and Sciences
DUNS #
City
Denver
State
CO
Country
United States
Zip Code
80208

  Publications

Hoofnagle, Andrew N; Stoner, James W; Lee, Thomas et al. (2004) Phosphorylation-dependent changes in structure and dynamics in ERK2 detected by SDSL and EPR. Biophys J 86:395-403
Harbridge, James R; Rinard, George A; Quine, Richard W et al. (2002) Enhanced signal intensities obtained by out-of-phase rapid-passage EPR for samples with long electron spin relaxation times. J Magn Reson 156:41-51
Harbridge, James R; Eaton, Sandra S; Eaton, Gareth R (2002) Electron spin-lattice relaxation in radicals containing two methyl groups, generated by gamma-irradiation of polycrystalline solids. J Magn Reson 159:195-206
Yong, L; Harbridge, J; Quine, R W et al. (2001) Electron spin relaxation of triarylmethyl radicals in fluid solution. J Magn Reson 152:156-61
Persson, M; Harbridge, J R; Hammarstrom, P et al. (2001) Comparison of electron paramagnetic resonance methods to determine distances between spin labels on human carbonic anhydrase II. Biophys J 80:2886-97
Huber, M; Lindgren, M; Hammarstrom, P et al. (2001) Phase memory relaxation times of spin labels in human carbonic anhydrase II: pulsed EPR to determine spin label location. Biophys Chem 94:245-56
Lemos, S S; Perille Collins, M L; Eaton, S S et al. (2000) Comparison of EPR-visible Cu(2+) sites in pMMO from Methylococcus capsulatus (Bath) and Methylomicrobium album BG8. Biophys J 79:1085-94
Zhou, Y; Bowler, B E; Lynch, K et al. (2000) Interspin distances in spin-labeled metmyoglobin variants determined by saturation recovery EPR. Biophys J 79:1039-52
Zhou, Y; Bowler, B E; Eaton, G R et al. (2000) Electron spin-lattice relaxation rates for high-spin Fe(III) complexes in glassy solvents at temperatures between 6 and 298 K. J Magn Reson 144:115-22
Eaton, G R; Eaton, S S (1999) Solvent and temperature dependence of spin echo dephasing for chromium(V) and vanadyl complexes in glassy solution. J Magn Reson 136:63-8

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