Abstract

The objectives of the proposed investigation are: 1) to obtain more information about lectin structure by studying such parameters as valence, specificity, subunit structure, and analyses of products produced by fragmentation and chemical modification; 2) to study the relationship of lectin structure to function by comparative studies of various lectins and their modified forms; and 3) to learn more about the natural role of lectins by following the development and distribution of a lectin during the life cycle of a plant. The Dolichos biflorus and wheat germ lectins are the principal lectins under investigation although the comparative aspects of this study will be extended to other lectins. In addition to yielding basic information about the structures and role of lectins, the information obtained in this investigation should enhance the flexibility and adaptation of these lectins for use in studies of complex carbohydrates and as probes for studying cell surface architecture.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM021882-12
Application #
3270776
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1978-02-01
Project End
1988-01-31
Budget Start
1986-02-01
Budget End
1987-01-31
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California Davis
Department
Type
Earth Sciences/Resources
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Roberts, Nicholas J; Morieri, Giulia; Kalsi, Gurpreet et al. (2013) Rhizobial and mycorrhizal symbioses in Lotus japonicus require lectin nucleotide phosphohydrolase, which acts upstream of calcium signaling. Plant Physiol 161:556-67
Kalsi, G; Etzler, M E (2000) Localization of a Nod factor-binding protein in legume roots and factors influencing its distribution and expression. Plant Physiol 124:1039-48
Hamelryck, T W; Loris, R; Bouckaert, J et al. (1999) Carbohydrate binding, quaternary structure and a novel hydrophobic binding site in two legume lectin oligomers from Dolichos biflorus. J Mol Biol 286:1161-77
Etzler, M E; Kalsi, G; Ewing, N N et al. (1999) A nod factor binding lectin with apyrase activity from legume roots. Proc Natl Acad Sci U S A 96:5856-61
Roberts, N J; Brigham, J; Wu, B et al. (1999) A Nod factor-binding lectin is a member of a distinct class of apyrases that may be unique to the legumes. Mol Gen Genet 262:261-7
Schnell, D J; Hori, K; Herrmann, S M et al. (1994) Biosynthesis of DB58 lectin in cell suspension cultures of Dolichos biflorus. Arch Biochem Biophys 310:229-35
Chao, Q; Etzler, M E (1994) Incorrect targeting of plant vacuolar lectins in yeast. J Biol Chem 269:20866-71
Etzler, M E (1994) Isolation and characterization of subunits of DB58, a lectin from the stems and leaves of Dolichos biflorus. Biochemistry 33:9778-83
Chao, Q; Casalongue, C; Quinn, J M et al. (1994) Expression and partial characterization of Dolichos biflorus seed lectin in Escherichia coli. Arch Biochem Biophys 313:346-50
Etzler, M E (1994) A comparison of the carbohydrate binding properties of two Dolichos biflorus lectins. Glycoconj J 11:395-9

Showing the most recent 10 out of 18 publications