The specificities of lectins for particular carbohydrate residues have led to their widespread use as tools in the characterization of complex carbohydrates and as probes for studying cell surface topography. Among their health related implications has been their use in blood typing, separation of populations of lymphocytes prior to bone marrow transplantation and various diagnostic assays. It is the long term objective of this research to obtain more information about the structures of lectins and their relationship to lectin function; such information should be valuable in allowing greater flexibility in the use of these tools as well as providing a better awareness of their limitations. This investigation will focus on the N-acetylgalactosamine specific lectins and will use the family of lectins from Dolichos biflorus as a model experimental system for exploring the relationship of structure to function in this class of lectins.
The specific aims of this proposal are: 1) to continue the structural characterization of these lectins, including a comparison of their primary structures and the characterization of a newly discovered phosphorylated form of one of these lectins; 2) to continue studies of lectin function and the localization of active sites on these proteins; and 3) to initiate site specific mutagenesis studies in and effort to extend our understanding of structure-function relationships. A variety of biochemical methods including recombinant DNA technology, peptide fractionation, photoaffinity labelling and immunochemical procedures will be used in this study.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM021882-14
Application #
3270773
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1978-02-01
Project End
1993-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
14
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of California Davis
Department
Type
Earth Sciences/Resources
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
Roberts, Nicholas J; Morieri, Giulia; Kalsi, Gurpreet et al. (2013) Rhizobial and mycorrhizal symbioses in Lotus japonicus require lectin nucleotide phosphohydrolase, which acts upstream of calcium signaling. Plant Physiol 161:556-67
Kalsi, G; Etzler, M E (2000) Localization of a Nod factor-binding protein in legume roots and factors influencing its distribution and expression. Plant Physiol 124:1039-48
Hamelryck, T W; Loris, R; Bouckaert, J et al. (1999) Carbohydrate binding, quaternary structure and a novel hydrophobic binding site in two legume lectin oligomers from Dolichos biflorus. J Mol Biol 286:1161-77
Etzler, M E; Kalsi, G; Ewing, N N et al. (1999) A nod factor binding lectin with apyrase activity from legume roots. Proc Natl Acad Sci U S A 96:5856-61
Roberts, N J; Brigham, J; Wu, B et al. (1999) A Nod factor-binding lectin is a member of a distinct class of apyrases that may be unique to the legumes. Mol Gen Genet 262:261-7
Schnell, D J; Hori, K; Herrmann, S M et al. (1994) Biosynthesis of DB58 lectin in cell suspension cultures of Dolichos biflorus. Arch Biochem Biophys 310:229-35
Chao, Q; Etzler, M E (1994) Incorrect targeting of plant vacuolar lectins in yeast. J Biol Chem 269:20866-71
Etzler, M E (1994) Isolation and characterization of subunits of DB58, a lectin from the stems and leaves of Dolichos biflorus. Biochemistry 33:9778-83
Chao, Q; Casalongue, C; Quinn, J M et al. (1994) Expression and partial characterization of Dolichos biflorus seed lectin in Escherichia coli. Arch Biochem Biophys 313:346-50
Etzler, M E (1994) A comparison of the carbohydrate binding properties of two Dolichos biflorus lectins. Glycoconj J 11:395-9

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