Abstract

The research proposed here seeks to determine the electronic structures of some iron containing enzymes using Mossbauer spectroscopy and electron paramagnetic resonance. The experiments include: 1) Further studies of the cluster structures and reaction mechanism of aconitase, a key enzyme in the citric acid cycle. 2) Continuation of research into the nature of the active sites of dioxygenases. These enzymes cleave catechols by oxygen insertion and are of major importance in the biodegradation of aromatic compounds in the biosphere. 3) Continuation of Mossbauer studies of cytochrome oxidase, the terminal enzyme of the respiratory chain. The a3-site of the enzyme, the locus of oxygen reduction, will be studied with enzymes isolated from beef heart mitochondria, Thermus thermophilus and Nitrosomonas europeae. 4) It is also proposed to study a lignin-degrading enzyme from the fungus P. chrysosporium.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM022701-12
Application #
3271255
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1976-03-01
Project End
1991-02-28
Budget Start
1987-03-01
Budget End
1988-02-29
Support Year
12
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Minnesota Twin Cities
Department
Type
Schools of Arts and Sciences
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455

  Publications

England, Jason; Farquhar, Erik R; Guo, Yisong et al. (2011) Characterization of a tricationic trigonal bipyramidal iron(IV) cyanide complex, with a very high reduction potential, and its iron(II) and iron(III) congeners. Inorg Chem 50:2885-96
Chakrabarti, Mrinmoy; Deng, Liang; Holm, R H et al. (2010) The modular nature of all-ferrous edge-bridged double cubanes. Inorg Chem 49:1647-50
Neibergall, Matthew B; Stubna, Audria; Mekmouche, Yasmina et al. (2007) Hydrogen peroxide dependent cis-dihydroxylation of benzoate by fully oxidized benzoate 1,2-dioxygenase. Biochemistry 46:8004-16
Klinker, Eric J; Jackson, Timothy A; Jensen, Michael P et al. (2006) A tosylimido analogue of a nonheme oxoiron(IV) complex. Angew Chem Int Ed Engl 45:7394-7
Kaizer, Jozsef; Klinker, Eric J; Oh, Na Young et al. (2004) Nonheme FeIVO complexes that can oxidize the C-H bonds of cyclohexane at room temperature. J Am Chem Soc 126:472-3
Roelfes, Gerard; Vrajmasu, Vladislav; Chen, Kui et al. (2003) End-on and side-on peroxo derivatives of non-heme iron complexes with pentadentate ligands: models for putative intermediates in biological iron/dioxygen chemistry. Inorg Chem 42:2639-53
Rohde, Jan-Uwe; In, Jun-Hee; Lim, Mi Hee et al. (2003) Crystallographic and spectroscopic characterization of a nonheme Fe(IV)-O complex. Science 299:1037-9
Lim, Mi Hee; Rohde, Jan-Uwe; Stubna, Audria et al. (2003) An FeIV=O complex of a tetradentate tripodal nonheme ligand. Proc Natl Acad Sci U S A 100:3665-70
Vrajmasu, Vladislav V; Bominaar, Emile L; Meyer, Jacques et al. (2002) Mossbauer study of reduced rubredoxin as purified and in whole cells. Structural correlation analysis of spin Hamiltonian parameters. Inorg Chem 41:6358-71
Andres, Hanspeter; Bominaar, Emile L; Smith, Jeremy M et al. (2002) Planar three-coordinate high-spin Fe(II) complexes with large orbital angular momentum: Mossbauer, electron paramagnetic resonance, and electronic structure studies. J Am Chem Soc 124:3012-25

Showing the most recent 10 out of 44 publications