Abstract

This proposal contains plans for a detailed biochemical study of eukaryotic tRNA gene expression and its control. Using cloned yeast tRNA genes the work will focus on the mechanism of their transcription and the characterization of the components of the transcription complex. Some of the enzymes involved in the conversion of the tRNA precursors to mature tRNA (e.g., RNase P, splicing endonuclease, tRNA modifying enzymes) will be cloned, purified and studied. The analysis of nonsense suppressor mutants of yeast will corroborate the biochemical results of this study.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM022854-12
Application #
3271379
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1976-03-01
Project End
1991-02-28
Budget Start
1987-03-01
Budget End
1988-02-29
Support Year
12
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Fu, Xian; Söll, Dieter; Sevostyanova, Anastasia (2018) Challenges of site-specific selenocysteine incorporation into proteins by Escherichia coli. RNA Biol 15:461-470
Umehara, Takuya; Kosono, Saori; Söll, Dieter et al. (2018) Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli. Genes (Basel) 9:
Kunjapur, Aditya M; Stork, Devon A; Kuru, Erkin et al. (2018) Engineering posttranslational proofreading to discriminate nonstandard amino acids. Proc Natl Acad Sci U S A 115:619-624
Mukai, Takahito; Sevostyanova, Anastasia; Suzuki, Tateki et al. (2018) [A facile method for producing selenocysteine-containing proteins]. Angew Chem Weinheim Bergstr Ger 130:7333-7337
Vargas-Rodriguez, Oscar; Englert, Markus; Merkuryev, Anna et al. (2018) Recoding of the selenocysteine UGA codon by cysteine in the presence of a non-canonical tRNACys and elongation factor SelB. RNA Biol 15:471-479
Ho, Joanne M; Bakkalbasi, Erol; Söll, Dieter et al. (2018) Drugging tRNA aminoacylation. RNA Biol 15:667-677
Mukai, Takahito; Sevostyanova, Anastasia; Suzuki, Tateki et al. (2018) A Facile Method for Producing Selenocysteine-Containing Proteins. Angew Chem Int Ed Engl 57:7215-7219
Reynolds, Noah M; Vargas-Rodriguez, Oscar; Söll, Dieter et al. (2017) The central role of tRNA in genetic code expansion. Biochim Biophys Acta Gen Subj 1861:3001-3008
Gan, Qinglei; Fan, Chenguang (2017) Increasing the fidelity of noncanonical amino acid incorporation in cell-free protein synthesis. Biochim Biophys Acta Gen Subj 1861:3047-3052
Bryson, David I; Fan, Chenguang; Guo, Li-Tao et al. (2017) Continuous directed evolution of aminoacyl-tRNA synthetases. Nat Chem Biol 13:1253-1260

Showing the most recent 10 out of 151 publications