Abstract

The catalytic properties, anomalous behavior, mechanism of action and important structural characteristics of homogeneous mammalian arylsulfatase enzymes will be studied. The chemical nature of turnover-modified aryl sulfatase A, a nearly inactive enzyme form which is produced during the catalytic process, will be examined in order to determine how the enzyme brings about is own inactivation. A novel, powerful and widely applicable method for the purification of most mammalian aryl sulfatase enzymes will be explored in detail. A sensitive assay for human urine aryl sulfatase will be developed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM022933-08
Application #
3271409
Study Section
Biochemistry Study Section (BIO)
Project Start
1976-06-01
Project End
1986-12-31
Budget Start
1985-01-01
Budget End
1986-12-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Purdue University
Department
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907

  Publications

Laidler, P M; Waheed, A; Van Etten, R L (1988) Arylsulfatase A from human tissues contains an endo-beta-N-acetylglucosaminidase F-resistant oligosaccharide. Acta Biochim Pol 35:343-56
Waheed, A; Van Etten, R L; Koob, R et al. (1988) Targeting of phosphomannosyl-deficient arylsulfatase A to lysosomes of I-cell fibroblasts. Eur J Cell Biol 45:262-7
Laidler, P M; Silberring, J; Van Etten, R L (1986) A modified radioimmunoassay for arylsulfatase A in human serum and urine. Clin Chim Acta 158:23-31
Laidler, P M; Waheed, A; Van Etten, R L (1985) Structural and immunochemical characterization of human urine arylsulfatase A purified by affinity chromatography. Biochim Biophys Acta 827:73-83
Waheed, A; Van Etten, R L (1985) Sulfhydryl groups of rabbit liver arylsulfatase A. Biochim Biophys Acta 831:67-73
Waheed, A; Risley, J M; Van Etten, R L (1985) Structural and immunological relationships among mammalian arylsulfatase A enzymes. Comp Biochem Physiol B 82:855-62
Waheed, A; van Etten, R L (1985) Phosphorylation and sulfation of arylsulfatase A accompanies biosynthesis of the enzyme in normal and carcinoma cell lines. Biochim Biophys Acta 847:53-61
Waheed, A; Van Etten, R L (1985) Biosynthesis and processing of lysosomal acid phosphatase in cultured human cells. Arch Biochem Biophys 243:274-83
Laidler, P M; Ryder, K W; Glick, M R et al. (1985) Radioimmunoassay for arylsulfatase A in urine. Clin Chem 31:391-6
Waheed, A; Van Etten, R L (1985) Purification of mammalian arylsulfatase A enzymes by subunit affinity chromatography. Int J Pept Protein Res 26:362-72