The thymidine analogue Bromodeoxyuridine has been shown to alter the redundancy of the ribosomal RNA cistrons of Drosophila. We are using the drug to study the regulatory events influencing the function of these genes. Genetic, cytological, microhybridization, and cloning techniques are being used to determine in which tissues and stages the BrdU effect is manifested, whether sequences surrounding the rDNAs are altered in frequency, whether the BrdU effect is related to or differs from compensatory and abo related mechanism which also alter rDNA redundancy, and whether any of these mechanisms selectively discriminate intron bearing sequences of the rDNA. Long-range goals will include the isolation of gene products regulating the rDNA functions, and the identification of the specific nucleotide sequences to which they bind.
| Goodrich-Young, C; Krider, H M (1989) Nucleolar dominance and replicative dominance in Drosophila interspecific hybrids. Genetics 123:349-58 |
| Slavicek, J M; Krider, H M (1987) The organization and composition of the ribosomal RNA gene non-transcribed spacer of D. busckii is unique among the drosophilids. Genet Res 50:173-80 |
| Dutton, F L; Krider, H M (1985) Expression and amplification of the genes for ribosomal RNA in bobbed mutants of Drosophila melanogaster. Genet Res 45:155-65 |
