Abstract

The yeast, Saccharomyces cerevisiae, will be used to determine how the chromosome assembles and is topologically altered during the cell cycle. The following distinct but related steps in formation of the dynamic chromosome will be studied. a) The cis- and trans-acting factors involved in controlling histone synthesis and stoichiometry in S phase will be identified. b) Strains in which individual core histones H2B and H4 are repressible by GAL promoters will be used to determine the steps in histone assembly, how nucleosomes segregate during DNA replication and whether gross changes in chromosome structure affect transcription. c) Deletions in the charged histone ends will also be analyzed by conditional synthesis to determine their effects on assembly of the nucleosome and changes in chromosome structure. d) Since N-terminal acetylation of histones has been implicated in assembly and structural changes of the chromosome, we will study this process by site-directed mutagenesis of acetylated lysines. Also, we will use a cloned gene (HAT-c), whose mutation results in decreased histone acetylase activity, to probe the function of acetylation during the yeast cell cycle.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM023674-15
Application #
3271828
Study Section
Molecular Biology Study Section (MBY)
Project Start
1977-01-01
Project End
1992-12-31
Budget Start
1991-01-01
Budget End
1991-12-31
Support Year
15
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Arts and Sciences
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Xu, Heng-hao; Su, Trent; Xue, Yong (2016) Histone H3 N-terminal acetylation sites especially K14 are important for rDNA silencing and aging. Sci Rep 6:21900
Xue, Yong; Vashisht, Ajay A; Tan, Yuliang et al. (2014) PRB1 is required for clipping of the histone H3 N terminal tail in Saccharomyces cerevisiae. PLoS One 9:e90496
Tan, Yuliang; Xue, Yong; Song, Chunying et al. (2013) Acetylated histone H3K56 interacts with Oct4 to promote mouse embryonic stem cell pluripotency. Proc Natl Acad Sci U S A 110:11493-8
Yu, Yongxin; Song, Chunying; Zhang, Qiongyi et al. (2012) Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA. Mol Cell 46:7-17
Kitada, Tasuku; Kuryan, Benjamin G; Tran, Nancy Nga Huynh et al. (2012) Mechanism for epigenetic variegation of gene expression at yeast telomeric heterochromatin. Genes Dev 26:2443-55
Sperling, Adam S; Jeong, Kyeong Soo; Kitada, Tasuku et al. (2011) Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast. Proc Natl Acad Sci U S A 108:12693-8
Chin, Mark H; Mason, Mike J; Xie, Wei et al. (2009) Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures. Cell Stem Cell 5:111-23
Xie, Wei; Song, Chunying; Young, Nicolas L et al. (2009) Histone h3 lysine 56 acetylation is linked to the core transcriptional network in human embryonic stem cells. Mol Cell 33:417-27
Houseley, Jonathan; Rubbi, Liudmilla; Grunstein, Michael et al. (2008) A ncRNA modulates histone modification and mRNA induction in the yeast GAL gene cluster. Mol Cell 32:685-95
Shahbazian, Mona D; Grunstein, Michael (2007) Functions of site-specific histone acetylation and deacetylation. Annu Rev Biochem 76:75-100

Showing the most recent 10 out of 48 publications