Abstract

This is a proposal to investigate how the animal-vegetal (A-V) axis is determined in the sea urchin embryo. A popular model is that the A-V axis is determined by the interaction of factors emanating from the animal and vegetal regions of the embryo. The Angerer laboratory studies the animal determinants, which are likely to be transcription factors arising from the egg that activate batteries of genes expressed in the animal region of the embryo. There are three objectives. The first objective is to determine whether there is a general mechanism that establishes the animal domain (non-vegetal transcription domain, or NTD) by cloning several factors that reside there. The second objective is to study the interactions between the animal and vegetal factors that establish a border between the domains. The third objective is to assess the role of BMP2/4 signaling in specifying the animal fates.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM025553-23
Application #
6385337
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Zatz, Marion M
Project Start
1978-07-01
Project End
2004-03-31
Budget Start
2001-04-01
Budget End
2002-03-31
Support Year
23
Fiscal Year
2001
Total Cost
$393,336
Indirect Cost

  Institution

Name
University of Rochester
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627

  Publications

Sethi, Aditya J; Angerer, Robert C; Angerer, Lynne M (2009) Gene regulatory network interactions in sea urchin endomesoderm induction. PLoS Biol 7:e1000029
Dunn, Ewan F; Moy, Vanessa N; Angerer, Lynne M et al. (2007) Molecular paleoecology: using gene regulatory analysis to address the origins of complex life cycles in the late Precambrian. Evol Dev 9:10-24
Angerer, Lynne M; Newman, Laurel A; Angerer, Robert C (2005) SoxB1 downregulation in vegetal lineages of sea urchin embryos is achieved by both transcriptional repression and selective protein turnover. Development 132:999-1008
Angerer, Lynne M; Angerer, Robert C (2003) Patterning the sea urchin embryo: gene regulatory networks, signaling pathways, and cellular interactions. Curr Top Dev Biol 53:159-98
Kenny, Alan P; Oleksyn, David W; Newman, Laurel A et al. (2003) Tight regulation of SpSoxB factors is required for patterning and morphogenesis in sea urchin embryos. Dev Biol 261:412-25
Kenny, A P; Angerer, L M; Angerer, R C (2001) SpSoxB1 serves an essential architectural function in the promoter SpAN, a tolloid/BMP1-related gene. Gene Expr 9:283-90
Howard, E W; Newman, L A; Oleksyn, D W et al. (2001) SpKrl: a direct target of beta-catenin regulation required for endoderm differentiation in sea urchin embryos. Development 128:365-75
Angerer, L M; Oleksyn, D W; Logan, C Y et al. (2000) A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis. Development 127:1105-14
Angerer, L M; Angerer, R C (2000) Animal-vegetal axis patterning mechanisms in the early sea urchin embryo. Dev Biol 218:12-Jan
Wardle, F C; Angerer, L M; Angerer, R C et al. (1999) Regulation of BMP signaling by the BMP1/TLD-related metalloprotease, SpAN. Dev Biol 206:63-72

Showing the most recent 10 out of 40 publications