Abstract

This research is aimed at understanding the mechanism of charge transfer across biological interfaces. This interfacial region of finite depth is of importance in biological processes which range from energy transduction to cell-cell interactions. The kinetics of selected photoinitiated charge transfer reactions localized at the interface are used as probes of this region of the lipid bilayer. The electric field dependence of the kinetics of charged hydrophobic pigments will be used to study properties polarity and dielectric gradients) of this region. Rigid donor-acceptor complexes specifically orientable at the bilayer-water interface will be synthesized and used to probe the interface on the short (ns) time scale. Proton transfers in this region will be followed by suitable donors or acceptors, e.g. quinones. The cross membrane transit time of both charged and uncharged species will be measured by these photoinitiated single interface charge transfer reactions and suitable redox, pH or ion gradients. The measurements are made with fast voltage and current amplifiers and very fast digitizers. The light sources are flash lamp and N2 laser pumped dye lasers.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM025693-09
Application #
3273257
Study Section
(SSS)
Project Start
1978-07-01
Project End
1988-02-29
Budget Start
1986-12-01
Budget End
1988-02-29
Support Year
9
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Hou, Harvey J M; Sakmar, Thomas P (2010) Methodology of pulsed photoacoustics and its application to probe photosystems and receptors. Sensors (Basel) 10:5642-67
Liu, Yan; Edens, Gregory J; Grzymski, Joseph et al. (2008) Volume and enthalpy changes of proton transfers in the bacteriorhodopsin photocycle studied by millisecond time-resolved photopressure measurements. Biochemistry 47:7752-61
Hou, Harvey J M; Mauzerall, David (2006) The A-Fx to F(A/B) step in synechocystis 6803 photosystem I is entropy driven. J Am Chem Soc 128:1580-6
Mauzerall, David; Liu, Yan; Edens, Gregory J et al. (2003) Measurement of enthalpy and volume changes in photoinitiated reactions on the ms timescale with a novel pressure cell. Photochem Photobiol Sci 2:788-90
Hou, J M; Boichenko, V A; Diner, B A et al. (2001) Thermodynamics of electron transfer in oxygenic photosynthetic reaction centers: volume change, enthalpy, and entropy of electron-transfer reactions in manganese-depleted photosystem II core complexes. Biochemistry 40:7117-25
Boichenko, V A; Hou, J M; Mauzerall, D (2001) Thermodynamics of electron transfer in oxygenic photosynthetic reaction centers: volume change, enthalpy, and entropy of electron-transfer reactions in the intact cells of the cyanobacterium Synechocystis PCC 6803. Biochemistry 40:7126-32
Hou, J M; Boichenko, V A; Wang, Y C et al. (2001) Thermodynamics of electron transfer in oxygenic photosynthetic reaction centers: a pulsed photoacoustic study of electron transfer in photosystem I reveals a similarity to bacterial reaction centers in both volume change and entropy. Biochemistry 40:7109-16
Sun, K; Mauzerall, D (1996) Evidence for ion chain mechanism of the nonlinear charge transport of hydrophobic ions across lipid bilayers. Biophys J 71:295-308
Sun, K; Mauzerall, D (1996) Charge transfer across a single lipid-water interface causes ion pumping across the bilayer. Biophys J 71:309-16
Sun, K; Mauzerall, D (1996) A simple light-driven transmembrane proton pump. Proc Natl Acad Sci U S A 93:10758-62

Showing the most recent 10 out of 27 publications