Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM025846-17
Application #
2174549
Study Section
Biochemistry Study Section (BIO)
Project Start
1980-04-01
Project End
1997-12-31
Budget Start
1996-04-01
Budget End
1997-12-31
Support Year
17
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
Public Health Research Institute
Department
Type
DUNS #
City
Newark
State
NY
Country
United States
Zip Code

  Publications

Cervera, J; Bendala, E; Britton, H G et al. (1996) Photoaffinity labeling with UMP of lysine 992 of carbamyl phosphate synthetase from Escherichia coli allows identification of the binding site for the pyrimidine inhibitor. Biochemistry 35:7247-55
Hong, J; Salo, W L; Lusty, C J et al. (1994) Carbamyl phosphate synthetase III, an evolutionary intermediate in the transition between glutamine-dependent and ammonia-dependent carbamyl phosphate synthetases. J Mol Biol 243:131-40
Bueso, J; Lusty, C J; Rubio, V (1994) Location of the binding site for the allosteric activator IMP in the COOH-terminal domain of Escherichia coli carbamyl phosphates synthetase. Biochem Biophys Res Commun 203:1083-9
Lusty, C J; Liao, M (1993) Substitution of Glu841 by lysine in the carbamate domain of carbamyl phosphate synthetase alters the catalytic properties of the glutaminase subunit. Biochemistry 32:1278-84
Cervera, J; Conejero-Lara, F; Ruiz-Sanz, J et al. (1993) The influence of effectors and subunit interactions on Escherichia coli carbamoyl-phosphate synthetase studied by differential scanning calorimetry. J Biol Chem 268:12504-11
Kern, C B; Lusty, C J; Davidson, J N (1992) Evidence that mammalian glutamine-dependent carbamyl phosphate synthetase arose through gene fusion. J Mol Evol 35:217-22
Guillou, F; Liao, M; Garcia-Espana, A et al. (1992) Mutational analysis of carbamyl phosphate synthetase. Substitution of Glu841 leads to loss of functional coupling between the two catalytic domains of the synthetase subunit. Biochemistry 31:1656-64
Lusty, C J (1992) Detection of an enzyme bound gamma-glutamyl acyl ester of carbamyl phosphate synthetase of Escherichia coli. FEBS Lett 314:135-8
Mullins, L S; Lusty, C J; Raushel, F M (1991) Alterations in the energetics of the carbamoyl phosphate synthetase reaction by site-directed modification of the essential sulfhydryl group. J Biol Chem 266:8236-40
Rubio, V; Cervera, J; Lusty, C J et al. (1991) Domain structure of the large subunit of Escherichia coli carbamoyl phosphate synthetase. Location of the binding site for the allosteric inhibitor UMP in the COOH-terminal domain. Biochemistry 30:1068-75

Showing the most recent 10 out of 18 publications