Abstract

The major objective of this proposal is the isolation and characterization of ribosomal DNA from Acanthamoeba castellanii. Recombinant DNA techniques will be used to isolate the gene, restriction endonuclease mapping and R-loop mapping will be used to determine the topology of the isolated DNA fragments. DNA sequencing of promotor and terminator regions will be carried out and compared to similar sequences determined in other eukaryotes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026059-06
Application #
3273519
Study Section
Genetics Study Section (GEN)
Project Start
1979-02-01
Project End
1985-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Colorado State University-Fort Collins
Department
Type
Schools of Arts and Sciences
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523

  Publications

Polakowski, Nicholas; Paule, Marvin R (2002) Purification and characterization of transcription factor IIIA from Acanthamoeba castellanii. Nucleic Acids Res 30:1977-84
Matthews, J L; Zwick, M G; Paule, M R (1995) Coordinate regulation of ribosomal component synthesis in Acanthamoeba castellanii: 5S RNA transcription is down regulated during encystment by alteration of TFIIIA activity. Mol Cell Biol 15:3327-35
Radebaugh, C A; Matthews, J L; Geiss, G K et al. (1994) TATA box-binding protein (TBP) is a constituent of the polymerase I-specific transcription initiation factor TIF-IB (SL1) bound to the rRNA promoter and shows differential sensitivity to TBP-directed reagents in polymerase I, II, and III transcription fac Mol Cell Biol 14:597-605
Imboden, M A; Matthews, J L; Lofquist, A K et al. (1992) An exonuclease requiring an intact helical stem for specificity produces the 3' end of Acanthamoeba castellanii 5 S RNA. J Biol Chem 267:24601-10
Perna, P J; Harris, G H; Iida, C T et al. (1992) The start site of the Acanthamoeba castellanii ribosomal RNA transcription unit. Gene Expr 2:71-8
Zwick, M G; Imboden, M A; Paule, M R (1991) Specific transcription of an Acanthamoeba castellanii 5S RNA gene in homologous nuclear extracts. Nucleic Acids Res 19:1681-6
Zwick, M G; Wiggs, M; Paule, M R (1991) Sequence and organization of 5S RNA genes from the eukaryotic protist Acanthamoeba castellanii. Gene 101:153-7
Paule, M R; Bateman, E; Hoffman, L et al. (1991) Initiation and regulation mechanisms of ribosomal RNA transcription in the eukaryote Acanthamoeba castellanii. Mol Cell Biochem 104:119-26
Kownin, P; Bateman, E; Paule, M R (1988) Effects of single-base substitutions within the Acanthamoeba castellanii rRNA promoter on transcription and on binding of transcription initiation factor and RNA polymerase I. Mol Cell Biol 8:747-53
Kownin, P; Bateman, E; Paule, M R (1987) Eukaryotic RNA polymerase I promoter binding is directed by protein contacts with transcription initiation factor and is DNA sequence-independent. Cell 50:693-9

Showing the most recent 10 out of 13 publications