Abstract

These studies will define the binding sites of regulatory proteins to the yeast cytochrome c genes, and identify regulatory proteins which bind specifically to these genes. The approaches to be used involve in vitro mutagenesis of the genes followed by transformation of yeast cells and selection for transformants having the mutant phenotype. In addition, a fused gene system involving the 5 feet sequence of the yeast CYC1 (iso-1-cytochrome c) gene and the structural region of the E. coli galK (galactokinase) gene will be used to allow selection of oxygen constitutive mutants. The precise location of the mutations obtained will be determined by DNA sequencing. The regulatory proteins will be identified and purified by the isolation of chromatin-complexed CYC1 plasmids from yeast cells. Those proteins bound specifically to the CYC1 DNA, and differentially bound to CYC1 DNA in cells grown under anaerobic and aerobic catabolite repressed and derepressed conditions, will be characterized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026061-07
Application #
3273529
Study Section
Genetics Study Section (GEN)
Project Start
1978-12-01
Project End
1987-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
State University of New York at Albany
Department
Type
Schools of Arts and Sciences
DUNS #
City
Albany
State
NY
Country
United States
Zip Code
12222

  Publications

Bose, Sohini; Dutko, James A; Zitomer, Richard S (2005) Genetic factors that regulate the attenuation of the general stress response of yeast. Genetics 169:1215-26
Klinkenberg, Lee G; Mennella, Thomas A; Luetkenhaus, Katharina et al. (2005) Combinatorial repression of the hypoxic genes of Saccharomyces cerevisiae by DNA binding proteins Rox1 and Mot3. Eukaryot Cell 4:649-60
Mennella, Thomas A; Klinkenberg, Lee G; Zitomer, Richard S (2003) Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein. Eukaryot Cell 2:1288-303
Khalaf, R A; Zitomer, R S (2001) The DNA binding protein Rfg1 is a repressor of filamentation in Candida albicans. Genetics 157:1503-12
Limbach, M P; Zitomer, R S (2000) The isolation and characterization of missense mutants in the general repressor protein Ssn6 of Saccharomyces cerevisiae. Mol Gen Genet 263:455-62
Kastaniotis, A J; Mennella, T A; Konrad, C et al. (2000) Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast. Mol Cell Biol 20:7088-98
Xin, H; Taudte, S; Kallenbach, N R et al. (2000) DNA binding by single HMG box model proteins. Nucleic Acids Res 28:4044-50
Deckert, J; Khalaf, R A; Hwang, S M et al. (1999) Characterization of the DNA binding and bending HMG domain of the yeast hypoxic repressor Rox1. Nucleic Acids Res 27:3518-26
Deckert, J; Torres, A M; Hwang, S M et al. (1998) The anatomy of a hypoxic operator in Saccharomyces cerevisiae. Genetics 150:1429-41
Zitomer, R S; Limbach, M P; Rodriguez-Torres, A M et al. (1997) Approaches to the study of Rox1 repression of the hypoxic genes in the yeast Saccharomyces cerevisiae. Methods 11:279-88

Showing the most recent 10 out of 15 publications