Abstract

The genetic maps of the enteric bacteria Salmonella and E. coli have remained remarkably similar despite the fact that these organisms have been separated for substantial evolutionary time and their sequence homology has diverged. This suggests that selective forces have prevented survival of cells that have undergone chromosome rearrangement. Despite evolutionary stability of the genome, many events are known that cause rearrangement of the chromosome. These include transposable elements and the events they induce, and spontaneous duplication and inversion events that seem to occur by recombination between repeated sequences that are separated in the chromosome. We will investigate these events by studying IS sequences and drug resistance transposons, and by devising genetic means of selecting and studying rearrangements. We'll study the mechanisms of rearrangements and the physiological consequences of these rearrangements. In particular, we will pursue the idea that gene duplication is an advantageous event for bacteria. We suspect that the structure of the bacterial chromosome includes sequences located appropriately for generation of advantageous duplications.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM027068-07
Application #
3274497
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1979-12-01
Project End
1990-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Arts and Sciences
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Maisnier-Patin, Sophie; Roth, John R (2018) Selection and Plasmid Transfer Underlie Adaptive Mutation in Escherichia coli. Genetics 210:821-841
Yamayoshi, Itsugo; Maisnier-Patin, Sophie; Roth, John R (2018) Selection-Enhanced Mutagenesis of lac Genes Is Due to Their Coamplification with dinB Encoding an Error-Prone DNA Polymerase. Genetics 208:1009-1021
Roth, John R; Maisnier-Patin, Sophie (2016) Reinterpreting Long-Term Evolution Experiments: Is Delayed Adaptation an Example of Historical Contingency or a Consequence of Intermittent Selection? J Bacteriol 198:1009-12
Maisnier-Patin, Sophie; Roth, John R (2015) The Origin of Mutants Under Selection: How Natural Selection Mimics Mutagenesis (Adaptive Mutation). Cold Spring Harb Perspect Biol 7:a018176
Reams, Andrew B; Roth, John R (2015) Mechanisms of gene duplication and amplification. Cold Spring Harb Perspect Biol 7:a016592
Sano, Emiko; Maisnier-Patin, Sophie; Aboubechara, John Paul et al. (2014) Plasmid copy number underlies adaptive mutability in bacteria. Genetics 198:919-33
Reams, Andrew B; Kofoid, Eric; Duleba, Natalie et al. (2014) Recombination and annealing pathways compete for substrates in making rrn duplications in Salmonella enterica. Genetics 196:119-35
Huseby, Douglas L; Roth, John R (2013) Evidence that a metabolic microcompartment contains and recycles private cofactor pools. J Bacteriol 195:2864-79
Näsvall, Joakim; Sun, Lei; Roth, John R et al. (2012) Real-time evolution of new genes by innovation, amplification, and divergence. Science 338:384-7
Reams, Andrew B; Kofoid, Eric; Kugelberg, Elisabeth et al. (2012) Multiple pathways of duplication formation with and without recombination (RecA) in Salmonella enterica. Genetics 192:397-415

Showing the most recent 10 out of 50 publications