Abstract

The objective of the project is to understand how gene activity is controlled in early development in sea urchin embryos and in mouse embryos. Using cloned genes for snRNAs we will determine the sequences required for correct developmental regulation of gene expression. We will use an in vivo system, microinjection of DNA into sea urchin embryos, and an in vitro s- dependent system which we have developed. Protein factors involved in transcription and 3' end formation of the sea urchin U1 RNA will be characterized and we will attempt to detect factors involved in developmental regulation. We will also determine the sequence requirements for correct tissue-specific expression of the mouse U1b genes. Additional sea urchin snRNA genes will be isolated and characterized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM027789-11
Application #
3275043
Study Section
Molecular Biology Study Section (MBY)
Project Start
1980-05-01
Project End
1993-06-30
Budget Start
1990-07-01
Budget End
1991-06-30
Support Year
11
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Florida State University
Department
Type
Schools of Arts and Sciences
DUNS #
020520466
City
Tallahassee
State
FL
Country
United States
Zip Code
32306

  Publications

Wagner, Eric J; Burch, Brandon D; Godfrey, Ashley C et al. (2007) A genome-wide RNA interference screen reveals that variant histones are necessary for replication-dependent histone pre-mRNA processing. Mol Cell 28:692-9
Wang, Z F; Ingledue, T C; Dominski, Z et al. (1999) Two Xenopus proteins that bind the 3' end of histone mRNA: implications for translational control of histone synthesis during oogenesis. Mol Cell Biol 19:835-45
Edelmann, L; Zheng, L; Wang, Z F et al. (1998) The TATA binding protein in the sea urchin embryo is maternally derived. Dev Biol 204:293-304
Ramamurthy, L; Ingledue, T C; Pilch, D R et al. (1996) Increasing the distance between the snRNA promoter and the 3' box decreases the efficiency of snRNA 3'-end formation. Nucleic Acids Res 24:4525-34
Bartlett, J S; Sethna, M; Ramamurthy, L et al. (1996) Efficient expression of protein coding genes from the murine U1 small nuclear RNA promoters. Proc Natl Acad Sci U S A 93:8852-7
Stefanovic, B; Marzluff, W F (1994) The proximal element is capable of determining proper temporal expression of the embryonic sea urchin U2 snRNA gene. Gene Expr 4:1-18
Li, J M; Parsons, R A; Marzluff, W F (1994) Transcription of the sea urchin U6 gene in vitro requires a TATA-like box, a proximal sequence element, and sea urchin USF, which binds an essential E box. Mol Cell Biol 14:2191-200
Williams, A S; Ingledue 3rd, T C; Kay, B K et al. (1994) Changes in the stem-loop at the 3' terminus of histone mRNA affects its nucleocytoplasmic transport and cytoplasmic regulation. Nucleic Acids Res 22:4660-6
Sakallah, S A; Norton, D R; Zhang, W et al. (1994) Isolation and characterization of the tandemly repeated U6 genes from the sea urchin Strongylocentrotus purpuratus. Biochim Biophys Acta 1218:439-42
Wendelburg, B J; Marzluff, W F (1992) Two promoter elements are necessary and sufficient for expression of the sea urchin U1 snRNA gene. Nucleic Acids Res 20:3743-51

Showing the most recent 10 out of 31 publications