Abstract

A major aim is to continue studies on the in vivo roles of eukaryotic DNA topoisomerases using yeast as a model system. Two new yeast topoisomerases, products of the TOP3 and UPRI genes, will be investigated, as will the functional overlap of these enzymes with the previously known DNA topoisomerase I. Suppresor genes that, when mutated, improve the growth of various topoisomerase mutants, will be cloned and analyzed. The gene(s) coding for the two forms of Xenopus DNA topoisomerase I will be cloned and used to study the developmentally regulated expression of these enzymes. A novel genetic system will be employed to study interactions among the various proteins involved in transcriptional repression of the yeast silent mating-type loci. The chromatin at these genes may be similar to heterochromatin in higher eukaryotes. Also, a new approach for finding yeast DNA replication genes will be employed. Finally, the gene for a yeast cruciform-cutting endonuclease will be investigated. This enzyme may be involved in resolving the branched recombination intermediate known as the Holliday junction. Therefore, the effect of mutations in this gene on recombination will be studied.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM028220-12
Application #
3275505
Study Section
Molecular Biology Study Section (MBY)
Project Start
1980-07-01
Project End
1995-06-30
Budget Start
1991-07-01
Budget End
1992-06-30
Support Year
12
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
Schools of Arts and Sciences
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Hsu, Hao-Chi; Wang, Chia-Lin; Wang, Mingzhu et al. (2013) Structural basis for allosteric stimulation of Sir2 activity by Sir4 binding. Genes Dev 27:64-73
Ren, Jie; Wang, Chia-Lin; Sternglanz, Rolf (2010) Promoter strength influences the S phase requirement for establishment of silencing at the Saccharomyces cerevisiae silent mating type Loci. Genetics 186:551-60
Sampath, Vinaya; Yuan, Peihua; Wang, Isabel X et al. (2009) Mutational analysis of the Sir3 BAH domain reveals multiple points of interaction with nucleosomes. Mol Cell Biol 29:2532-45
Krichevsky, Alexander; Gutgarts, Helen; Kozlovsky, Stanislav V et al. (2007) C2H2 zinc finger-SET histone methyltransferase is a plant-specific chromatin modifier. Dev Biol 303:259-69
Vaquero, Alejandro; Scher, Michael B; Lee, Dong Hoon et al. (2006) SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis. Genes Dev 20:1256-61
Connelly, Jessica J; Yuan, Peihua; Hsu, Hao-Chi et al. (2006) Structure and function of the Saccharomyces cerevisiae Sir3 BAH domain. Mol Cell Biol 26:3256-65
Zappulla, David C; Maharaj, Arindel S R; Connelly, Jessica J et al. (2006) Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs. BMC Mol Biol 7:40
Xiang, Song; Kim, Eun Young; Connelly, Jessica J et al. (2006) The crystal structure of Cdc42 in complex with collybistin II, a gephyrin-interacting guanine nucleotide exchange factor. J Mol Biol 359:35-46
Liu, Bingsheng; Sutton, Ann; Sternglanz, Rolf (2005) A yeast polyamine acetyltransferase. J Biol Chem 280:16659-64
Wang, Xiaorong; Connelly, Jessica J; Wang, Chia-Lin et al. (2004) Importance of the Sir3 N terminus and its acetylation for yeast transcriptional silencing. Genetics 168:547-51

Showing the most recent 10 out of 35 publications