Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM028575-15A1
Application #
2175218
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1980-12-01
Project End
1999-11-30
Budget Start
1995-12-15
Budget End
1996-11-30
Support Year
15
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Stalder, Elizabeth S; Nagy, Lauren H; Batalla, Pilar et al. (2011) The epitope for the polyol-responsive monoclonal antibody 8RB13 is in the flap-domain of the beta-subunit of bacterial RNA polymerase and can be used as an epitope tag for immunoaffinity chromatography. Protein Expr Purif 77:26-33
Zhao, Kai; Liu, Mingzhu; Burgess, Richard R (2010) Promoter and regulon analysis of nitrogen assimilation factor, sigma54, reveal alternative strategy for E. coli MG1655 flagellar biosynthesis. Nucleic Acids Res 38:1273-83
Thompson, Nancy E; Glaser, Bryan T; Foley, Katherine M et al. (2009) Minimal promoter systems reveal the importance of conserved residues in the B-finger of human transcription factor IIB. J Biol Chem 284:24754-66
Glaser, Bryan T; Bergendahl, Veit; Anthony, Larry C et al. (2009) Studying the salt dependence of the binding of sigma70 and sigma32 to core RNA polymerase using luminescence resonance energy transfer. PLoS One 4:e6490
Glaser, Bryan T; Bergendahl, Veit; Thompson, Nancy E et al. (2007) LRET-based HTS of a small-compound library for inhibitors of bacterial RNA polymerase. Assay Drug Dev Technol 5:759-68
Zhao, Kai; Liu, Mingzhu; Burgess, Richard R (2007) Adaptation in bacterial flagellar and motility systems: from regulon members to 'foraging'-like behavior in E. coli. Nucleic Acids Res 35:4441-52
Probasco, Mitchell D; Thompson, Nancy E; Burgess, Richard R (2007) Immunoaffinity purification and characterization of RNA polymerase from Shewanella oneidensis. Protein Expr Purif 55:23-30
Lamberski, Jennifer A; Thompson, Nancy E; Burgess, Richard R (2006) Expression and purification of a single-chain variable fragment antibody derived from a polyol-responsive monoclonal antibody. Protein Expr Purif 47:82-92
Sabree, Zakee L; Bergendahl, Veit; Liles, Mark R et al. (2006) Identification and characterization of the gene encoding the Acidobacterium capsulatum major sigma factor. Gene 376:144-51
Thompson, Nancy E; Jensen, Debra Bridges; Lamberski, Jennifer A et al. (2006) Purification of protein complexes by immunoaffinity chromatography: application to transcription machinery. Genet Eng (N Y) 27:81-100

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