Abstract

We are interested in the post-transcriptional mechanisms by which E. coli controls the levels of specific proteins. We will study the structural features of mRNAs that can be accommodated by ribosomes during the initiation step. We will study the direct role of the initiator tRNA by asking if mutant tRNAs can replace the initiator. We will study the role during initiation of tRNAs reading the codon 3' to the initiation codon. We will study two translational repressors, one of which inspects several mRNAs in the initiation domain, apparently by directly recognizing primary sequences. We will study an E. coli mRNA that may bind to ribosomes using non-standard complementarity to 16S ribosomal RNA. We will probe the structure of the track within which mRNAs sit in the ribosome during initiation, using a new technology that should reveal all rRNA domains that surround the message.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028685-08
Application #
3275948
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1980-12-01
Project End
1990-11-30
Budget Start
1987-12-01
Budget End
1988-11-30
Support Year
8
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Colorado at Boulder
Department
Type
Schools of Arts and Sciences
DUNS #
City
Boulder
State
CO
Country
United States
Zip Code
80309

  Publications

Morris, K N; Jensen, K B; Julin, C M et al. (1998) High affinity ligands from in vitro selection: complex targets. Proc Natl Acad Sci U S A 95:2902-7
Ringquist, S; Gold, L (1998) Toeprinting assays. Mapping by blocks to reverse transcriptase primer extension. Methods Mol Biol 77:283-95
Chen, H; McBroom, D G; Zhu, Y Q et al. (1996) Inhibitory RNA ligand to reverse transcriptase from feline immunodeficiency virus. Biochemistry 35:6923-30
Jayasena, V K; Brown, D; Shtatland, T et al. (1996) In vitro selection of RNA specifically cleaved by bacteriophage T4 RegB endonuclease. Biochemistry 35:2349-56
He, Y Y; Stockley, P G; Gold, L (1996) In vitro evolution of the DNA binding sites of Escherichia coli methionine repressor, MetJ. J Mol Biol 255:55-66
Chen, H; Brown, D; Gold, L (1996) Novel methods of generating specific oligonucleotide inhibitors of viral polymerases. Methods Enzymol 275:503-20
Binkley, J; Allen, P; Brown, D M et al. (1995) RNA ligands to human nerve growth factor. Nucleic Acids Res 23:3198-205
Brown, D; Gold, L (1995) Selection and characterization of RNAs replicated by Q beta replicase. Biochemistry 34:14775-82
Brown, D; Gold, L (1995) Template recognition by an RNA-dependent RNA polymerase: identification and characterization of two RNA binding sites on Q beta replicase. Biochemistry 34:14765-74
Allen, P; Worland, S; Gold, L (1995) Isolation of high-affinity RNA ligands to HIV-1 integrase from a random pool. Virology 209:327-36

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