The further design, study, and bioanalytical applications of novel polymer membrane based chemical sensors are proposed. This research will build upon the recent successes of this program in developing a variety of new ion/gas chemical sensors that are useful for the direct detection of biomedically important species in complex samples, including whole blood. Efforts during the next phase of this project will include both fundamental and applied studies, with an emphasis on pursuing completely new concepts/strategies, both with respect the type analyte ions that may be detected via polymer membrane technology, and the chemical methods used to achieve desired ion selectively. A major thrust of the program will be to identify and study highly selective membrane chemistries for in situ sensing of anionic species. Specific new goals include: 1) examining the response mechanism and potential bioanalytical applications of a novel polymer membrane electrode that exhibits unique and clinically useful potentiometric response to the anticoagulant heparin, a macromolecular polyanion (both porcine and beef types); 2) studying the response properties/mechanisms of metalloporphyrin base anion-selective membranes with focus on determining the theoretical and practical advantages of adding fixed lipophilic anionic sites to enhance anion selectivity and on developing a Co(III)-porphyrin or corrin based nitrite selective sensors for monitoring cellular activity of nitric oxide synthase; and 3) assessing the utility of new molecular/ion imprinting techniques to prepare polymer films (silicone rubber) that may exhibit enhanced potentiometric selectivity to biologically important anions, especially carbonate, phosphates, low molecular weight heparin fragments, etc. In addition to these new directions, ongoing efforts to use various surface modification methods to render polymer membrane types gas and ion-selective catheter type sensors more blood compatible will be extended into this next phase of support. It is anticipated that this research program will continue to provide the biomedical community with an array of new/or improved chemical sensors as well the basic knowledge and chemistry that will be required to develop additional sensors, both electrochemical and optical, in the future.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028882-16
Application #
2391887
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1981-04-01
Project End
1998-03-31
Budget Start
1997-04-01
Budget End
1998-03-31
Support Year
16
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Pietrzak, Mariusz; Meyerhoff, Mark E; Malinowska, Elzbieta (2007) Polymeric membrane electrodes with improved fluoride selectivity and lifetime based on Zr(IV)- and Al(III)-tetraphenylporphyrin derivatives. Anal Chim Acta 596:201-9
Zhang, Wei; Rozniecka, Ewa; Malinowska, Elzbieta et al. (2002) Optical chloride sensor based on dimer-monomer equilibrium of indium(III) octaethylporphyrin in polymeric film. Anal Chem 74:4548-57
Ye, Q; Meyerhoff, M E (2001) Rotating electrode potentiometry: lowering the detection limits of nonequilibrium polyion-sensitive membrane electrodes. Anal Chem 73:332-6
Dai, S; Ye, Q; Wang, E et al. (2000) Optical detection of polycations via polymer film-modified microtiter plates: response mechanism and bioanalytical applications. Anal Chem 72:3142-9
Steinle, E D; Amemiya, S; Buhlmann, P et al. (2000) Origin of non-Nernstian anion response slopes of metalloporphyrin-based liquid/polymer membrane electrodes. Anal Chem 72:5766-73
Dai, S; Esson, J M; Lutze, O et al. (1999) Bioanalytical applications of polyion-sensitive electrodes. J Pharm Biomed Anal 19:1-14
Chang, L C; Meyerhoff, M E; Yang, V C (1999) Electrochemical assay of plasminogen activators in plasma using polyion-sensitive membrane electrode detection. Anal Biochem 276:8-12
Ramamurthy, N; Baliga, N; Wakefield, T W et al. (1999) Determination of low-molecular-weight heparins and their binding to protamine and a protamine analog using polyion-sensitive membrane electrodes. Anal Biochem 266:116-24
Malinowska, E; Meyerhoff, M E (1998) Influence of nonionic surfactants on the potentiometric response of ion-selective polymeric membrane electrodes designed for blood electrolyte measurements. Anal Chem 70:1477-88
Meruva, R K; Meyerhoff, M E (1998) Catheter-type sensor for potentiometric monitoring of oxygen, pH and carbon dioxide. Biosens Bioelectron 13:201-12

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