Abstract

The determination of new forms of base modification in nucleic acids is an essential step in understanding structure-function relationships. New nucleosides will be isolated and their structures established, from transfer RNA of archaebacteria, E. coli, rat and beef liver, mouse L-M cells, and Chlorella pyrenoidosa. Structural studies will be carried out on modified bases from the bacteriophage RL38J1. The primary methods employed will be high performance liquid chromatography and mass spectrometry, both separately and directly combined.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM029812-12
Application #
3277474
Study Section
(BET)
Project Start
1978-05-01
Project End
1990-04-30
Budget Start
1986-05-01
Budget End
1987-04-30
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Pharmacy
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Guymon, Rebecca; Pomerantz, Steven C; Ison, J Nicholas et al. (2007) Post-transcriptional modifications in the small subunit ribosomal RNA from Thermotoga maritima, including presence of a novel modified cytidine. RNA 13:396-403
Guymon, Rebecca; Pomerantz, Steven C; Crain, Pamela F et al. (2006) Influence of phylogeny on posttranscriptional modification of rRNA in thermophilic prokaryotes: the complete modification map of 16S rRNA of Thermus thermophilus. Biochemistry 45:4888-99
Del Campo, Mark; Recinos, Claudia; Yanez, Giscard et al. (2005) Number, position, and significance of the pseudouridines in the large subunit ribosomal RNA of Haloarcula marismortui and Deinococcus radiodurans. RNA 11:210-9
Cleary, Michael D; Meiering, Christopher D; Jan, Eric et al. (2005) Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay. Nat Biotechnol 23:232-7
Chen, Peng; Crain, Pamela F; Nasvall, S Joakim et al. (2005) A ""gain of function"" mutation in a protein mediates production of novel modified nucleosides. EMBO J 24:1842-51
Pomerantz, Steven C; McCloskey, James A (2005) Detection of the common RNA nucleoside pseudouridine in mixtures of oligonucleotides by mass spectrometry. Anal Chem 77:4687-97
McCloskey, James A; Rozenski, Jef (2005) The Small Subunit rRNA Modification Database. Nucleic Acids Res 33:D135-8
Zhou, Shaolian; Sitaramaiah, Devarasetty; Noon, Kathleen R et al. (2004) Structures of two new ""minimalist"" modified nucleosides from archaeal tRNA. Bioorg Chem 32:82-91
Zhou, Shaolian; Sitaramaiah, Devarasetty; Pomerantz, Steven C et al. (2004) Tandem mass spectrometry for structure assignments of wye nucleosides from transfer RNA. Nucleosides Nucleotides Nucleic Acids 23:41-50
Douthwaite, Stephen; Crain, Pamela F; Liu, Mingfu et al. (2004) The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748. J Mol Biol 337:1073-7

Showing the most recent 10 out of 67 publications