Abstract

Posttranscriptional modifications in RNA serve numerous functions, including stabilization of tertiary structure, and enhancement of the fidelity of intermolecular interactions. The determination of new natural forms of modification in nucleic acids is an essential first step in understanding such structure-function relationships. Transfer and ribosomal RNA will be isolated and screened by combined liquid chromatography-mass spectrometry to determine the presence and structures of modified nucleosides. The structures of new nucleosides will be determined by mass spectrometry, NMR, and additional appropriate methods. In the case of tRNA emphasis will be placed on thermophilic archaebacteria, and the potential correlation of modifications with phylogenetic status and with structure stabilization at high temperature. Mass spectrometry with electrophoresis for sequence location of modified nucleosides in 16S ribosomal RNA. The structures of liposidomycins, nucleoside antibiotic inhibitors of bacterial cell wall biosynthesis, will be determined, primarily by tandem mass spectrometry.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM029812-16
Application #
3277473
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1978-05-01
Project End
1995-04-30
Budget Start
1990-05-01
Budget End
1991-04-30
Support Year
16
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Pharmacy
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Guymon, Rebecca; Pomerantz, Steven C; Ison, J Nicholas et al. (2007) Post-transcriptional modifications in the small subunit ribosomal RNA from Thermotoga maritima, including presence of a novel modified cytidine. RNA 13:396-403
Guymon, Rebecca; Pomerantz, Steven C; Crain, Pamela F et al. (2006) Influence of phylogeny on posttranscriptional modification of rRNA in thermophilic prokaryotes: the complete modification map of 16S rRNA of Thermus thermophilus. Biochemistry 45:4888-99
Del Campo, Mark; Recinos, Claudia; Yanez, Giscard et al. (2005) Number, position, and significance of the pseudouridines in the large subunit ribosomal RNA of Haloarcula marismortui and Deinococcus radiodurans. RNA 11:210-9
Cleary, Michael D; Meiering, Christopher D; Jan, Eric et al. (2005) Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay. Nat Biotechnol 23:232-7
Chen, Peng; Crain, Pamela F; Nasvall, S Joakim et al. (2005) A ""gain of function"" mutation in a protein mediates production of novel modified nucleosides. EMBO J 24:1842-51
Pomerantz, Steven C; McCloskey, James A (2005) Detection of the common RNA nucleoside pseudouridine in mixtures of oligonucleotides by mass spectrometry. Anal Chem 77:4687-97
McCloskey, James A; Rozenski, Jef (2005) The Small Subunit rRNA Modification Database. Nucleic Acids Res 33:D135-8
Zhou, Shaolian; Sitaramaiah, Devarasetty; Noon, Kathleen R et al. (2004) Structures of two new ""minimalist"" modified nucleosides from archaeal tRNA. Bioorg Chem 32:82-91
Zhou, Shaolian; Sitaramaiah, Devarasetty; Pomerantz, Steven C et al. (2004) Tandem mass spectrometry for structure assignments of wye nucleosides from transfer RNA. Nucleosides Nucleotides Nucleic Acids 23:41-50
Douthwaite, Stephen; Crain, Pamela F; Liu, Mingfu et al. (2004) The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748. J Mol Biol 337:1073-7

Showing the most recent 10 out of 67 publications