The described project continues to examine control of ion channels by intracellular pathways. Experiments will be conducted to understand the molecular and mechanistic details of the activation pathway leading from the 7-helix receptors to G-proteins and to the inwardly rectifying KGA potassium channel. Problems to be addressed include: the identity of the G-protein subunits involved, the nature of activation by Gs-coupled receptors; the relationship between permeation, magnesium block and slow gating; and stoichiometry of the assembled channel. Using the oocyte preparation, methods will be developed and exploited for controlling the cytoplasmic surface (perfused and injected oocytes) and for single channel (excised inside-out patches) experiments. Mammalian systems will be developed for heterologous expression of KGA. Biochemical experiments will study direct binding of labelled tracer amounts of appropriate segments of KGA (including intracellular regions produced by in vitro translation) with biochemical quantities of G-protein subunits attached to solid supports in order to identify regions on KGA and the specified G-protein subunits that interact. GTPase activating characteristics of the KGA channel will be addressed, as will the identity of the inwardly rectifying K-channel that is inhibited by a G-protein pathway in oocytes expressing rat brain mRNA. The applicant indicates that the channel to be studied is important in the control of heartbeat, analgesia, and in drug abuse.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM029836-17
Application #
2608786
Study Section
Special Emphasis Panel (ZRG1-NLS-1 (02))
Project Start
1981-04-01
Project End
1998-11-30
Budget Start
1997-12-01
Budget End
1998-11-30
Support Year
17
Fiscal Year
1998
Total Cost
Indirect Cost
Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125
Lester, Henry A; Miwa, Julie M; Srinivasan, Rahul (2012) Psychiatric drugs bind to classical targets within early exocytotic pathways: therapeutic effects. Biol Psychiatry 72:907-15
Kovoor, Abraham; Seyffarth, Petra; Ebert, Jana et al. (2005) D2 dopamine receptors colocalize regulator of G-protein signaling 9-2 (RGS9-2) via the RGS9 DEP domain, and RGS9 knock-out mice develop dyskinesias associated with dopamine pathways. J Neurosci 25:2157-65
Rahman, Zia; Schwarz, Johannes; Gold, Stephen J et al. (2003) RGS9 modulates dopamine signaling in the basal ganglia. Neuron 38:941-52
Petersson, E James; Brandt, Gabriel S; Zacharias, Niki M et al. (2003) Caging proteins through unnatural amino acid mutagenesis. Methods Enzymol 360:258-73
Rozengurt, Nora; Lopez, Ivan; Chiu, Chi-Sung et al. (2003) Time course of inner ear degeneration and deafness in mice lacking the Kir4.1 potassium channel subunit. Hear Res 177:71-80
Tong, Y; Brandt, G S; Li, M et al. (2001) Tyrosine decaging leads to substantial membrane trafficking during modulation of an inward rectifier potassium channel. J Gen Physiol 117:103-18
Li, M; Lester, H A (2001) Ion channel diseases of the central nervous system. CNS Drug Rev 7:214-40
Neusch, C; Rozengurt, N; Jacobs, R E et al. (2001) Kir4.1 potassium channel subunit is crucial for oligodendrocyte development and in vivo myelination. J Neurosci 21:5429-38
Maurer, J A; Elmore, D E; Lester, H A et al. (2000) Comparing and contrasting Escherichia coli and Mycobacterium tuberculosis mechanosensitive channels (MscL). New gain of function mutations in the loop region. J Biol Chem 275:22238-44
Kofuji, P; Ceelen, P; Zahs, K R et al. (2000) Genetic inactivation of an inwardly rectifying potassium channel (Kir4.1 subunit) in mice: phenotypic impact in retina. J Neurosci 20:5733-40

Showing the most recent 10 out of 64 publications