Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM030327-15
Application #
2175758
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1981-07-01
Project End
1997-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
15
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Ohio State University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210
Kubiak, R J; Yue, X; Hondal, R J et al. (2001) Involvement of the Arg-Asp-His catalytic triad in enzymatic cleavage of the phosphodiester bond. Biochemistry 40:5422-32
Kravchuk, A V; Zhao, L; Kubiak, R J et al. (2001) Mechanism of phosphatidylinositol-specific phospholipase C: origin of unusually high nonbridging thio effects. Biochemistry 40:5433-9
Hondal, R J; Zhao, Z; Kravchuk, A V et al. (1998) Mechanism of phosphatidylinositol-specific phospholipase C: a unified view of the mechanism of catalysis. Biochemistry 37:4568-80
Bruzik, K S; Nyholm, P G (1997) NMR study of the conformation of galactocerebroside in bilayers and solution: galactose reorientation during the metastable-stable gel transition. Biochemistry 36:566-75
Hondal, R J; Riddle, S R; Kravchuk, A V et al. (1997) Phosphatidylinositol-specific phospholipase C: kinetic and stereochemical evidence for an interaction between arginine-69 and the phosphate group of phosphatidylinositol. Biochemistry 36:6633-42
Huang, B; Schaeffer, C J; Li, Q et al. (1996) Splase: a new class IIS zinc-finger restriction endonuclease with specificity for Sp1 binding sites. J Protein Chem 15:481-9
Bruzik, K S; Hakeem, A A; Tsai, M D (1994) Are D- and L-chiro-phosphoinositides substrates of phosphatidylinositol-specific phospholipase C? Biochemistry 33:8367-74
Bruzik, K S; Morocho, A M; Jhon, D Y et al. (1992) Phospholipids chiral at phosphorus. Stereochemical mechanism for the formation of inositol 1-phosphate catalyzed by phosphatidylinositol-specific phospholipase C. Biochemistry 31:5183-93
Bruzik, K S; Tsai, M D (1991) Phospholipase stereospecificity at phosphorus. Methods Enzymol 197:258-69
Lin, G L; Bennett, C F; Tsai, M D (1990) Phospholipids chiral at phosphorus. Stereochemical mechanism of reactions catalyzed by phosphatidylinositide-specific phospholipase C from Bacillus cereus and guinea pig uterus. Biochemistry 29:2747-57

Showing the most recent 10 out of 20 publications