Abstract

Flagella of Chlamydomonas and other unicellular green algae are being studied to determine the functions of the individual flagellar components in the control and coordination of flagellar movement, and to learn more about the mechanisms involved in axonemal assembly and the control of flagellar growth. Chlamydomonas mutants defective in the control of flagellar waveform are being studied by light and electron microscopy and one-dimensional and two-dimensional gel electrophoresis in order to identify and localize the components involved in the regulation of flagellar waveform. Studies are being carried out to determine the location of calmodulin within the flagellar axoneme of Chlamydomonas and to investigate its possible involvement in the Ca ions-control of axonemal bend symmetry. The marine alga Micromonas pusilla is being studied by light and electron microscopy to learn more about the action of the central pair of microtubules during flagellar movement. The times and rates of synthesis of individual axonemal proteins in Chlamydomonas, their times of transport into the flagellum during flagellar regeneration, and whether they undergo processing during their assembly into the axonemal superstructure are being investigated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM030626-04
Application #
3278430
Study Section
Molecular Biology Study Section (MBY)
Project Start
1981-08-01
Project End
1985-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Worcester Foundation for Biomedical Research
Department
Type
DUNS #
City
Shrewsbury
State
MA
Country
United States
Zip Code

  Publications

Kubo, Tomohiro; Hirono, Masafumi; Aikawa, Takumi et al. (2015) Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Mol Biol Cell 26:2810-22
San Agustin, Jovenal T; Pazour, Gregory J; Witman, George B (2015) Intraflagellar transport is essential for mammalian spermiogenesis but is absent in mature sperm. Mol Biol Cell 26:4358-72
Yang, T Tony; Su, Jimmy; Wang, Won-Jing et al. (2015) Superresolution Pattern Recognition Reveals the Architectural Map of the Ciliary Transition Zone. Sci Rep 5:14096
Awata, Junya; Takada, Saeko; Standley, Clive et al. (2014) NPHP4 controls ciliary trafficking of membrane proteins and large soluble proteins at the transition zone. J Cell Sci 127:4714-27
Johnson, Eric A; Rice, Selena L; Preimesberger, Matthew R et al. (2014) Characterization of THB1, a Chlamydomonas reinhardtii truncated hemoglobin: linkage to nitrogen metabolism and identification of lysine as the distal heme ligand. Biochemistry 53:4573-89
Owa, Mikito; Furuta, Akane; Usukura, Jiro et al. (2014) Cooperative binding of the outer arm-docking complex underlies the regular arrangement of outer arm dynein in the axoneme. Proc Natl Acad Sci U S A 111:9461-6
Brown, Jason M; Witman, George B (2014) Cilia and Diseases. Bioscience 64:1126-1137
Lechtreck, Karl F; Brown, Jason M; Sampaio, Julio L et al. (2013) Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase. J Cell Biol 201:249-61
Brown, Jason M; Dipetrillo, Christen G; Smith, Elizabeth F et al. (2012) A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus. J Cell Sci 125:3904-13
Yang, Yong; Cochran, Deborah A; Gargano, Mary D et al. (2011) Regulation of flagellar motility by the conserved flagellar protein CG34110/Ccdc135/FAP50. Mol Biol Cell 22:976-87

Showing the most recent 10 out of 67 publications