Abstract

RNA is a ubiquitous biomolecule, whose structural complexity and activities are crucial to all cells. Detailed biochemical study of this molecule spans greater than or equal to 40 years, yet the variety of biological functions attributed to RNA has grown notably in recent times. Accordingly, a current list of RNA functions would likely be incomplete, and the biomedical sciences require an expanded list of possible cellular roles for RNA. This grant proposes, based on earlier results from this project, studies which potentially find new structures and functions for RNA's repertoire: which characterize the ability of RNA to fold into specific sites for small ligands such as amino acids, fatty acids, and ions. A newly discovered RNA catalyst which labilizes 5' terminal triphosphate will be characterized and exploited. New types of RNA catalysts for acyl transfer, and new RNA metalloenzymes will be sought.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM030881-16A1
Application #
2608793
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1982-07-01
Project End
2002-03-31
Budget Start
1998-04-01
Budget End
1999-03-31
Support Year
16
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Colorado at Boulder
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
City
Boulder
State
CO
Country
United States
Zip Code
80309

  Publications

Janas, Teresa; Janas, Tadeusz; Yarus, Michael (2012) Human tRNA(Sec) associates with HeLa membranes, cell lipid liposomes, and synthetic lipid bilayers. RNA 18:2260-8
Smit, Sandra; Yarus, Michael; Knight, Rob (2006) Natural selection is not required to explain universal compositional patterns in rRNA secondary structure categories. RNA 12:1-14
Janas, Tadeusz; Janas, Teresa; Yarus, Michael (2006) Specific RNA binding to ordered phospholipid bilayers. Nucleic Acids Res 34:2128-36
Knight, Rob; De Sterck, Hans; Markel, Rob et al. (2005) Abundance of correctly folded RNA motifs in sequence space, calculated on computational grids. Nucleic Acids Res 33:5924-35
Janas, Teresa; Janas, Tadeusz; Yarus, Michael (2004) A membrane transporter for tryptophan composed of RNA. RNA 10:1541-9
Janas, Tadeusz; Yarus, Michael (2003) Visualization of membrane RNAs. RNA 9:1353-61
Jadhav, Vasant R; Yarus, Michael (2002) Coenzymes as coribozymes. Biochimie 84:877-88
Illangasekare, Mali; Yarus, Michael (2002) Phenylalanine-binding RNAs and genetic code evolution. J Mol Evol 54:298-311
Jadhav, Vasant R; Yarus, Michael (2002) Acyl-CoAs from coenzyme ribozymes. Biochemistry 41:723-9
Kumar, R K; Yarus, M (2001) RNA-catalyzed amino acid activation. Biochemistry 40:6998-7004

Showing the most recent 10 out of 56 publications