Abstract

Work in our laboratory is primarily in the area of glycoprotein synthesis. We have made the rather surprising discovery that a single lipid-linked oligosaccharide serves as the general precursor of all asparagine-linked glycopeptides. We have studied the pathway of synthesis of this lipid linked oligosaccharide and have examined the regulation of its synthesis by pulse-chase experiments. We have also investigated the intracellular site of synthesis of the oligosaccharide and the site of its transfer to nascent polypeptide chains. In addition, we have considered the processing reactions that occur to glycoproteins following their synthesis. Known glycoprotein processing reactions include polypeptide chain cleavage, addition of fatty acyl groups, and processing of oligosaccharide carbohydrate. Finally, changes in glycosylation patterns associated with expression of particular cell surface antigens, with developmental changes in Dictyostelium, and with transformation have also been investigated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM031318-23
Application #
3279277
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1978-02-01
Project End
1988-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
23
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code

  Publications

Haserick, John R; Leon, Deborah R; Samuelson, John et al. (2017) Asparagine-Linked Glycans of Cryptosporidium parvum Contain a Single Long Arm, Are Barely Processed in the Endoplasmic Reticulum (ER) or Golgi, and Show a Strong Bias for Sites with Threonine. Mol Cell Proteomics 16:S42-S53
Bandini, Giulia; Haserick, John R; Motari, Edwin et al. (2016) O-fucosylated glycoproteins form assemblies in close proximity to the nuclear pore complexes of Toxoplasma gondii. Proc Natl Acad Sci U S A 113:11567-11572
Chatterjee, Aparajita; Ratner, Daniel M; Ryan, Christopher M et al. (2015) Anti-Retroviral Lectins Have Modest Effects on Adherence of Trichomonas vaginalis to Epithelial Cells In Vitro and on Recovery of Tritrichomonas foetus in a Mouse Vaginal Model. PLoS One 10:e0135340
Samuelson, John; Robbins, Phillips W (2015) Effects of N-glycan precursor length diversity on quality control of protein folding and on protein glycosylation. Semin Cell Dev Biol 41:121-8
Bushkin, G Guy; Motari, Edwin; Carpentieri, Andrea et al. (2013) Evidence for a structural role for acid-fast lipids in oocyst walls of Cryptosporidium, Toxoplasma, and Eimeria. MBio 4:e00387-13
Samuelson, John; Bushkin, G Guy; Chatterjee, Aparajita et al. (2013) Strategies to discover the structural components of cyst and oocyst walls. Eukaryot Cell 12:1578-87
Bushkin, G Guy; Motari, Edwin; Magnelli, Paula et al. (2012) ýý-1,3-glucan, which can be targeted by drugs, forms a trabecular scaffold in the oocyst walls of Toxoplasma and Eimeria. MBio 3:
Samuelson, John; Robbins, Phillips (2011) A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia. Trends Parasitol 27:17-22
Chatterjee, Anirban; Banerjee, Sulagna; Steffen, Martin et al. (2010) Evidence for mucin-like glycoproteins that tether sporozoites of Cryptosporidium parvum to the inner surface of the oocyst wall. Eukaryot Cell 9:84-96
Mitra, Sanghamitra; Cui, Jike; Robbins, Phillips W et al. (2010) A deeply divergent phosphoglucomutase (PGM) of Giardia lamblia has both PGM and phosphomannomutase activities. Glycobiology 20:1233-40

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