Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM031704-15
Application #
2176272
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1995-09-03
Project End
1999-08-31
Budget Start
1996-09-01
Budget End
1997-08-31
Support Year
15
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Allegheny University of Health Sciences
Department
Biochemistry
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19129
Kommoju, Phaneeswara-Rao; Chen, Zhi-wei; Bruckner, Robert C et al. (2011) Probing oxygen activation sites in two flavoprotein oxidases using chloride as an oxygen surrogate. Biochemistry 50:5521-34
Bruckner, Robert C; Winans, Jennifer; Jorns, Marilyn Schuman (2011) Pleiotropic impact of a single lysine mutation on biosynthesis of and catalysis by N-methyltryptophan oxidase. Biochemistry 50:4949-62
Jorns, Marilyn Schuman; Chen, Zhi-Wei; Mathews, F Scott (2010) Structural characterization of mutations at the oxygen activation site in monomeric sarcosine oxidase . Biochemistry 49:3631-9
Zhao, Guohua; Bruckner, Robert C; Jorns, Marilyn Schuman (2008) Identification of the oxygen activation site in monomeric sarcosine oxidase: role of Lys265 in catalysis. Biochemistry 47:9124-35
Hassan-Abdallah, Alshaimaa; Zhao, Guohua; Chen, Zhi-wei et al. (2008) Arginine 49 is a bifunctional residue important in catalysis and biosynthesis of monomeric sarcosine oxidase: a context-sensitive model for the electrostatic impact of arginine to lysine mutations. Biochemistry 47:2913-22
Hassan-Abdallah, Alshaimaa; Zhao, Guohua; Jorns, Marilyn Schuman (2008) Covalent flavinylation of monomeric sarcosine oxidase: identification of a residue essential for holoenzyme biosynthesis. Biochemistry 47:1136-43
Zhao, Gouhua; Jorns, Marilyn Schuman (2006) Spectral and kinetic characterization of the michaelis charge transfer complex in monomeric sarcosine oxidase. Biochemistry 45:5985-92
Chen, Zhi-wei; Hassan-Abdulah, Alshaimaa; Zhao, Gouhua et al. (2006) Heterotetrameric sarcosine oxidase: structure of a diflavin metalloenzyme at 1.85 A resolution. J Mol Biol 360:1000-18
Hassan-Abdallah, Alshaimaa; Zhao, Guohua; Jorns, Marilyn Schuman (2006) Role of the covalent flavin linkage in monomeric sarcosine oxidase. Biochemistry 45:9454-62
Hynson, Robert M G; Mathews, F Scott; Schuman Jorns, Marilyn (2006) Identification of a stable flavin-thiolate adduct in heterotetrameric sarcosine oxidase. J Mol Biol 362:656-63

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