Earlier work supported by this grant developed the chemistry and structural physics of protein hydrogen exchange (HX) and showed, given this fundamental information, how HX methods can be used to measure protein structure, stability, dynamics, binding, structure change, and folding. These studies led to the discovery of the previously unsuspected foldon dimension of protein structure. Results in our lab and others now suggest that proteins fold by putting cooperative units of the native structure (foldons) into place in a predetermined sequential manner, with prior structure acting as a template to support the formation and docking of incoming native-like foldon units. It also appears that proteins can use their foldons to control other functions including the rate of ligand binding and release and the stability and kinetics of structure change processes. This application proposes to further investigate the implications of the newly discovered foldon dimension of protein structure for protein folding and function.
Aims are as follows. (1) Continue work on foldons and folding in the major Staphylococcal Nuclease model. (2) Investigate the folding behavior of larger proteins, using maltose binding protein (41 kDa) as an initial model. (3) Study the role of transient partially unfolded states in other protein functions, with initial work directed at the Mad2 mitotic checkpoint protein.
These Aims will exploit the same powerful suite of integrated hydrogen exchange methods that were developed and used successfully in earlier studies together with newly developed fast 3D NMR and enhanced HX/mass spectrometry methods.

Public Health Relevance

Hydrogen exchange knowledge and methods developed in our earlier work have led to the discovery of a whole new dimension of protein structure and function based on cooperative foldon units. Foldons appear to provide the key to the protein folding and misfolding problem and to underlie some other protein functions as well. In order to understand the fundamental roles of protein molecules in health and disease, the experimentation proposed here aims to explore the implications of the newly discovered foldon dimension of protein structure for protein folding and misfolding and for protein function more broadly.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM031847-29
Application #
8318150
Study Section
Macromolecular Structure and Function B Study Section (MSFB)
Program Officer
Wehrle, Janna P
Project Start
1983-12-01
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
29
Fiscal Year
2012
Total Cost
$307,354
Indirect Cost
$109,354
Name
University of Pennsylvania
Department
Biochemistry
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Ye, Xiang; Mayne, Leland; Kan, Zhong-Yuan et al. (2018) Folding of maltose binding protein outside of and in GroEL. Proc Natl Acad Sci U S A 115:519-524
Nguyen, David; Mayne, Leland; Phillips, Michael C et al. (2018) Reference Parameters for Protein Hydrogen Exchange Rates. J Am Soc Mass Spectrom 29:1936-1939
Tischer, Alexander; Machha, Venkata R; Frontroth, Juan P et al. (2017) Enhanced Local Disorder in a Clinically Elusive von Willebrand Factor Provokes High-Affinity Platelet Clumping. J Mol Biol 429:2161-2177
Chetty, Palaniappan S; Mayne, Leland; Lund-Katz, Sissel et al. (2017) Helical structure, stability, and dynamics in human apolipoprotein E3 and E4 by hydrogen exchange and mass spectrometry. Proc Natl Acad Sci U S A 114:968-973
Mayne, Leland (2016) Hydrogen Exchange Mass Spectrometry. Methods Enzymol 566:335-56
Hu, Wenbing; Kan, Zhong-Yuan; Mayne, Leland et al. (2016) Cytochrome c folds through foldon-dependent native-like intermediates in an ordered pathway. Proc Natl Acad Sci U S A 113:3809-14
Englander, S Walter; Mayne, Leland; Kan, Zhong-Yuan et al. (2016) Protein Folding-How and Why: By Hydrogen Exchange, Fragment Separation, and Mass Spectrometry. Annu Rev Biophys 45:135-52
Casina, Veronica C; Hu, Wenbing; Mao, Jian-Hua et al. (2015) High-resolution epitope mapping by HX MS reveals the pathogenic mechanism and a possible therapy for autoimmune TTP syndrome. Proc Natl Acad Sci U S A 112:9620-5
Englander, S Walter; Mayne, Leland (2014) The nature of protein folding pathways. Proc Natl Acad Sci U S A 111:15873-80
Chetty, Palaniappan Sevugan; Nguyen, David; Nickel, Margaret et al. (2013) Comparison of apoA-I helical structure and stability in discoidal and spherical HDL particles by HX and mass spectrometry. J Lipid Res 54:1589-97

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