Abstract

Development of techniques using wide-bore, high-field (300-500 MHz), high-resolution NMR spectroscopy to study metabolic processes in living systems and in vitro will be undertaken. The use of multiply labeled substrates (13C, 15N, 2H) prepared by regiospecific synthesis will be of value in assigning chemical shifts to unknown and short-lived species. It is proposed to use cryoenzymology and flow cells to study the flux of carbon, hydrogen and nitrogen in biochemical reactions, to identify hitherto undetected species and to devise non-invasive methods for the analysis of microgram quantities of biologically important molecules during metabolism in mammalian, bacterial and plant cells, as well as whole, perfused organs and insects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM032596-03
Application #
3281595
Study Section
(SSS)
Project Start
1983-03-01
Project End
1988-02-29
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Texas A&M University
Department
Type
Schools of Arts and Sciences
DUNS #
City
College Station
State
TX
Country
United States
Zip Code
77845

  Publications

Ahmed, A A; Balboul, B A; Scott, A I et al. (1998) Eudesmane derivatives from Iva frutescens. Phytochemistry 47:411-3
Huang, K; Huang, Q l; Scott, A I (1998) Overexpression, single-step purification, and site-directed mutagenetic analysis of casbene synthase. Arch Biochem Biophys 352:144-52
Moyna, G; Williams, H J; Scott, A I et al. (1997) Conformational studies of paclitaxel analogs modified at the C-2' position in hydrophobic and hydrophilic solvent systems. J Med Chem 40:3305-11
Ahmed, A A; Abd el-Razek, M H; Abu Mostafa, E A et al. (1996) A new derivative of glucose and 2-C-methyl-D-erythritol from Ferula sinaica. J Nat Prod 59:1171-3
Roessner, C A; Scott, A I (1995) Fluorescence-based method for selection of recombinant plasmids. Biotechniques 19:760-4
Moore, M; Narasimhan, T R; Wang, X et al. (1993) Interaction of 2,3,7,8-tetrachlorodibenzo-p-dioxin, 12-O-tetradecanoylphorbol-13-acetate (TPA) and 17 beta-estradiol in MCF-7 human breast cancer cells. J Steroid Biochem Mol Biol 44:251-61
Ahmed, A A; Mahmoud, A A; Williams, H J et al. (1993) New sesquiterpene alpha-methylene lactones from the Egyptian plant Jasonia candicans. J Nat Prod 56:1276-80
Dominguez, X A; Sanchez, H; Garcia, S et al. (1992) Isolation and identification of xochitloldione and isoxochitlolone from Cnidosculus urens. J Nat Prod 55:221-4
Ortiz, C; Tellier, C; Williams, H et al. (1991) Diastereotopic covalent binding of the natural inhibitor leupeptin to trypsin: detection of two interconverting hemiacetals by solution and solid-state NMR spectroscopy. Biochemistry 30:10026-34
Dominguez, X A; Garcia, S; Williams, H J et al. (1989) Kukulkanins A and B, new chalcones from Mimosa tenuefolia. J Nat Prod 52:864-7

Showing the most recent 10 out of 16 publications