Abstract

Photosystem II (PSII) is an integral membrane protein complex in which photochemistry, electron and proton transport and catalysis of water oxidation and quinone reduction are facilitated and regulated by multiple ion cofactors and prosthetic groups, and the protein/lipid environment. The long-term objective of this project is to develop an understanding at the molecular level of the structure and assembly of the tetranuclear Mn active site of water oxidation, the catalytic mechanism of water oxidation, the electron-transfer properties of PSII, and the damaging side reactions that occur in the process of water oxidation. In the proposed studies, electron paramagnetic resonance spectroscopy will be used to study the assembly, coordination and redox chemistry, and the intermediate oxidation states of the tetranuclear Mn complex. These studies will take advantage of a newly-developed class of redox-active herbicides that will be used to

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM032715-09
Application #
3281783
Study Section
Molecular and Cellular Biophysics Study Section (BBCA)
Project Start
1984-08-01
Project End
1996-07-31
Budget Start
1992-08-01
Budget End
1993-07-31
Support Year
9
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Arts and Sciences
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Young, Karin J; Brennan, Bradley J; Tagore, Ranitendranath et al. (2015) Photosynthetic water oxidation: insights from manganese model chemistry. Acc Chem Res 48:567-74
Blakemore, James D; Hull, Jonathan F; Crabtree, Robert H et al. (2012) Aqueous speciation and electrochemical properties of a water-soluble manganese phthalocyanine complex. Dalton Trans 41:7681-8
McConnell, Iain L; Grigoryants, Vladimir M; Scholes, Charles P et al. (2012) EPR-ENDOR characterization of (17O, 1H, 2H) water in manganese catalase and its relevance to the oxygen-evolving complex of photosystem II. J Am Chem Soc 134:1504-12
Rivalta, Ivan; Brudvig, Gary W; Batista, Victor S (2012) Oxomanganese complexes for natural and artificial photosynthesis. Curr Opin Chem Biol 16:11-8
Rivalta, Ivan; Amin, Muhamed; Luber, Sandra et al. (2011) Structural-functional role of chloride in photosystem II. Biochemistry 50:6312-5
Luber, Sandra; Rivalta, Ivan; Umena, Yasufumi et al. (2011) S1-state model of the O2-evolving complex of photosystem II. Biochemistry 50:6308-11
Wei, Zi; Cady, Clyde W; Brudvig, Gary W et al. (2011) Photodamage of a Mn(III/IV)-oxo mixed-valence compound and photosystem II: evidence that a high-valent manganese species is responsible for UV-induced photodamage of the oxygen-evolving complex in photosystem II. J Photochem Photobiol B 104:118-25
Wang, Ting; Brudvig, Gary; Batista, Victor S (2010) Characterization of proton coupled electron transfer in a biomimetic oxomanganese complex: Evaluation of the DFT B3LYP level of theory. J Chem Theory Comput 6:755-760
McConnell, Iain; Li, Gonghu; Brudvig, Gary W (2010) Energy conversion in natural and artificial photosynthesis. Chem Biol 17:434-47
Wang, Ting; Brudvig, Gary W; Batista, Victor S (2010) Study of Proton Coupled Electron Transfer in a Biomimetic Dimanganese Water Oxidation Catalyst with Terminal Water Ligands. J Chem Theory Comput 6:2395-2401

Showing the most recent 10 out of 93 publications