Abstract

Resonance Raman and CARS (Coherent Anti-Stokes Raman Scattering) spectroscopic studies are planned for heme proteins, with a view toward elucidating the structural bases of biological function. Mechanisms of oxygen binding (hemoglobin and myoglobin), oxygen activation (cytochrome P450) and electron transport (cytochrome c oxidase) will be major foci. Model heme compounds will be used as a basis for interpreting the protein spectra. Time-resolved Raman studies of reaction intermediates will be initiated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM033576-16
Application #
3283463
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1979-04-01
Project End
1989-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
16
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Princeton University
Department
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code

  Publications

Spiro, Thomas G; Soldatova, Alexandra V; Balakrishnan, Gurusamy (2013) CO, NO and O2 as Vibrational Probes of Heme Protein Interactions. Coord Chem Rev 257:511-527
Kabil, Omer; Weeks, Colin L; Carballal, Sebastian et al. (2011) Reversible heme-dependent regulation of human cystathionine ýý-synthase by a flavoprotein oxidoreductase. Biochemistry 50:8261-3
Ibrahim, Mohammed; Derbyshire, Emily R; Marletta, Michael A et al. (2010) Probing soluble guanylate cyclase activation by CO and YC-1 using resonance Raman spectroscopy. Biochemistry 49:3815-23
Soldatova, Alexandra V; Ibrahim, Mohammed; Olson, John S et al. (2010) New light on NO bonding in Fe(III) heme proteins from resonance Raman spectroscopy and DFT modeling. J Am Chem Soc 132:4614-25
Ibrahim, Mohammed; Derbyshire, Emily R; Soldatova, Alexandra V et al. (2010) Soluble guanylate cyclase is activated differently by excess NO and by YC-1: resonance Raman spectroscopic evidence. Biochemistry 49:4864-71
Weeks, Colin L; Singh, Sangita; Madzelan, Peter et al. (2009) Heme regulation of human cystathionine beta-synthase activity: insights from fluorescence and Raman spectroscopy. J Am Chem Soc 131:12809-16
Singh, Sangita; Madzelan, Peter; Stasser, Jay et al. (2009) Modulation of the heme electronic structure and cystathionine beta-synthase activity by second coordination sphere ligands: The role of heme ligand switching in redox regulation. J Inorg Biochem 103:689-97
Derbyshire, Emily R; Gunn, Alexander; Ibrahim, Mohammed et al. (2008) Characterization of two different five-coordinate soluble guanylate cyclase ferrous-nitrosyl complexes. Biochemistry 47:3892-9
Xu, Changliang; Ibrahim, Mohammed; Spiro, Thomas G (2008) DFT analysis of axial and equatorial effects on heme-CO vibrational modes: applications to CooA and H-NOX heme sensor proteins. Biochemistry 47:2379-87
Spiro, Thomas (2008) A twist on heme signaling. ACS Chem Biol 3:673-5

Showing the most recent 10 out of 53 publications