Abstract

We have identified three genes whose cognate RNAs are growth regulated and are inducible in quiescent cells by platelet-derived growth factor and serum. Although these three genes are inducible by PDGF or serum, their response to other mitogenic agents varies. In addition, they are all inducible in the presence of high concentrations of cycloheximide (which means that they are primary responders), they are transcriptionally regulated, and they appear to be overexpressed in certain forms of human myeloid leukemias. They constitute, therefore, an interesting set of genes in which one could study the regulation of expression of growth regulated genes. The purpose of this application is to extend our previous studies on these three growth regulated genes and, more specifically, to characterize them structurally and to identify their regulatory elements. Once their regulatory elements are identified we hope to be able to characterize them both structurally and functionally. While this characterization is in progress we will also attempt to obtain in bacteria their respective proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM033694-04
Application #
3283607
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1984-07-01
Project End
1992-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Temple University
Department
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19122

  Publications

Valentinis, B; Navarro, M; Zanocco-Marani, T et al. (2000) Insulin receptor substrate-1, p70S6K, and cell size in transformation and differentiation of hemopoietic cells. J Biol Chem 275:25451-9
Dews, M; Prisco, M; Peruzzi, F et al. (2000) Domains of the insulin-like growth factor I receptor required for the activation of extracellular signal-regulated kinases. Endocrinology 141:1289-300
Gatzka, M; Prisco, M; Baserga, R (2000) Stabilization of the Ras oncoprotein by the insulin-like growth factor 1 receptor during anchorage-independent growth. Cancer Res 60:4222-30
Valentinis, B; Romano, G; Peruzzi, F et al. (1999) Growth and differentiation signals by the insulin-like growth factor 1 receptor in hemopoietic cells are mediated through different pathways. J Biol Chem 274:12423-30
Reiss, K; Yumet, G; Shan, S et al. (1999) Synthetic peptide sequence from the C-terminus of the insulin-like growth factor-I receptor that induces apoptosis and inhibition of tumor growth. J Cell Physiol 181:124-35
Morrione, A; Plant, P; Valentinis, B et al. (1999) mGrb10 interacts with Nedd4. J Biol Chem 274:24094-9
Baserga, R; Morrione, A (1999) Differentiation and malignant transformation: two roads diverged in a wood. J Cell Biochem Suppl 32-33:68-75
Prisco, M; Romano, G; Peruzzi, F et al. (1999) Insulin and IGF-I receptors signaling in protection from apoptosis. Horm Metab Res 31:80-9
Peruzzi, F; Prisco, M; Dews, M et al. (1999) Multiple signaling pathways of the insulin-like growth factor 1 receptor in protection from apoptosis. Mol Cell Biol 19:7203-15
Valentinis, B; Morrione, A; Peruzzi, F et al. (1999) Anti-apoptotic signaling of the IGF-I receptor in fibroblasts following loss of matrix adhesion. Oncogene 18:1827-36

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