Ribonuclease P (RNase P) cleaves pre-tRNAs to give the mature 5' termini. RNase P holoenzymes from Bacteria, Archaea and Eucarya have been shown to contain essential RNA subunits of similar size and structure, although there are pronounced differences in the size, number and contribution of the protein components. RNase P is unusual among the known nucleolytic RNAs in that a single enzyme recognizes a large number of substrates through tertiary contacts, rather than Watson-Crick base pairing. The purpose of the proposed research is to investigate how Saccharomyces cerevisiae nuclear RNase P recognizes and cleaves its substrates and how this holoenzyme interacts with the nuclear processing pathway for tRNA primary transcripts. In the previous period, this laboratory proposed secondary and tertiary structure models for the yeast nuclear RNase P RNA. The function of highly conserved sequences and structures in the RNA models were tested by directed mutagenesis and characterization of the resulting holoenzyme assembled in vivo. To aid in biochemical characterization, we purified the nuclear RNase and identified a complex subunit structure consisting of nine proteins tightly associated with the RNA subunit. All subunits are shown to be essential for life and for RNase P function in vivo, and eight of the nine protein subunits are also associated with RNase MRP, a related endonuclease involved in -re-ribosomal RNA processing in nucleoli. This close relationship to RNase MRP caused us to examine whether RNase P might also be nucleolar, leading to the discovery that much of the pre-tRNA processing pathway, including RNase P, is located in the nucleolus. Future work will proceed from the hypothesis that a limited number of the protein subunits of RNase P participate directly with the RNA subunit in pre-tRNA cleavage, while the majority of the enzyme protein content confers functions required in the complex nuclear environment. In this proposal, several specific questions will be addressed concerning the structure and function of yeast nuclear RNase P. [1] How are RNase P substrates recognized by the RNA and protein components of this complex holoenzyme? [2] How are the subunits of the holoenzyme fit together to achieve this function? [3] What are the other roles of the protein subunits in physiological function of the holoenzyme within the nuclear tRNA processing pathway?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034869-14
Application #
6180426
Study Section
Biochemistry Study Section (BIO)
Program Officer
Rhoades, Marcus M
Project Start
1985-04-01
Project End
2003-03-31
Budget Start
2000-04-01
Budget End
2001-03-31
Support Year
14
Fiscal Year
2000
Total Cost
$314,414
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Biochemistry
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Marvin, Michael C; Clauder-Munster, Sandra; Walker, Scott C et al. (2011) Accumulation of noncoding RNA due to an RNase P defect in Saccharomyces cerevisiae. RNA 17:1441-50
Walker, Scott C; Good, Paul D; Gipson, Theresa A et al. (2011) The dual use of RNA aptamer sequences for affinity purification and localization studies of RNAs and RNA-protein complexes. Methods Mol Biol 714:423-44
Marvin, Michael C; Walker, Scott C; Fierke, Carol A et al. (2011) Binding and cleavage of unstructured RNA by nuclear RNase P. RNA 17:1429-40
Srisawat, Chatchawan; Engelke, David R (2010) Selection of RNA aptamers that bind HIV-1 LTR DNA duplexes: strand invaders. Nucleic Acids Res 38:8306-15
Hopper, Anita K; Pai, Dave A; Engelke, David R (2010) Cellular dynamics of tRNAs and their genes. FEBS Lett 584:310-7
Coughlin, Daniel J; Babak, Tomas; Nihranz, Chad et al. (2009) Prediction and verification of mouse tRNA gene families. RNA Biol 6:195-202
Marvin, Michael C; Engelke, David R (2009) RNase P: increased versatility through protein complexity? RNA Biol 6:40-2
Hsieh, John; Walker, Scott C; Fierke, Carol A et al. (2009) Pre-tRNA turnover catalyzed by the yeast nuclear RNase P holoenzyme is limited by product release. RNA 15:224-34
Marvin, Michael C; Engelke, David R (2009) Broadening the mission of an RNA enzyme. J Cell Biochem 108:1244-51
Coughlin, Daniel J; Pleiss, Jeffrey A; Walker, Scott C et al. (2008) Genome-wide search for yeast RNase P substrates reveals role in maturation of intron-encoded box C/D small nucleolar RNAs. Proc Natl Acad Sci U S A 105:12218-23

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