Abstract

Radiofrequency pulse shaping has previously been shown to give enhanced magnetic resonance images and improved suppression of the water signal in nuclear magnetic resonance spectroscopy of small molecules in solution. We propose to extend this technique to study glucagon, whose aggregation dynamics are analogous to protein folding, and to study the actin:profilin complex, which plays a central role in muscle contraction. We will modify an existing commercial high field NMR to make pulse shaping routinely available. We will also, in a collaborative effort, demonstrate improved bandwidth and reduced amplifier dead time for Fourier transform electron paramagentic resonance. Finally, we ill continue our computational efforts to develop more useful shapes, and apply a new theoretical formalism to make quantitative predictions about directions for further improvement.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM035253-04
Application #
3287688
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1985-07-01
Project End
1992-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
4
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Princeton University
Department
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544

  Publications

Zhang, H; Lizitsa, N; Bryant, R G et al. (2001) Experimental characterization of intermolecular multiple-quantum coherence pumping efficiency in solution NMR. J Magn Reson 148:200-8
Lin, Y; Ahn, S; Murali, N et al. (2000) High-resolution, >1 GHz NMR in unstable magnetic fields. Phys Rev Lett 85:3732-5
Lin, Y Y; Lisitza, N; Ahn, S et al. (2000) Resurrection of crushed magnetization and chaotic dynamics in solution NMR spectroscopy. Science 290:118-22
Rizi, R R; Ahn, S; Alsop, D C et al. (2000) Intermolecular zero-quantum coherence imaging of the human brain. Magn Reson Med 43:627-32
Richter, W; Richter, M; Warren, W S et al. (2000) Functional magnetic resonance imaging with intermolecular multiple-quantum coherences. Magn Reson Imaging 18:489-94
Garrett-Roe, S; Warren, W S (2000) Numerical studies of intermolecular multiple quantum coherences: high-resolution NMR in inhomogeneous fields and contrast enhancement in MRI. J Magn Reson 146:13-Jan
Warren, W S; Ahn, S; Mescher, M et al. (1998) MR imaging contrast enhancement based on intermolecular zero quantum coherences. Science 281:247-51
Ahn, S; Warren, W S; Lee, S (1997) Quantum treatment of intermolecular multiple-quantum coherences with intramolecular J coupling in solution NMR. J Magn Reson 128:114-29
Vathyam, S; Lee, S; Warren, W S (1996) Homogeneous NMR spectra in inhomogeneous fields. Science 272:92-6
Richter, W; Lee, S; Warren, W S et al. (1995) Imaging with intermolecular multiple-quantum coherences in solution nuclear magnetic resonance. Science 267:654-7

Showing the most recent 10 out of 12 publications