Abstract

This is a revised continuation proposal. The broad objectives are to develop applications of gradients and radiofrequency pulse shaping for nuclear resonance spectroscopy of biomolecules, particularly in concentrated solvents. The focus is on intermolecular multiple-quantum (iMQ) coherences and crosspeaks between proteins and water. Both the theoretical and experimental work will be continued on dilute solution of biomolecules in water. Specifically, the in vivo imaging and spectroscopy work will be extended with the aims of enhancing image contrast and validating proposed mechanisms for functional MRI. High resolution NMR at high fields will be pursued using magnets which give inhomogeneous field (e.g. the existing 27T and proposed 30T magnets at the NHMFL). Work on small-signal amplification by solute-solvent coherence transfer will also be pursued with the view of revolutionizing the way in which low-gamma or low-concentration species are detected in NMR. Suppression of iMQ coherences in sequences such TOCSY will be explored to produce improved versions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM035253-11A1
Application #
2022054
Study Section
Molecular and Cellular Biophysics Study Section (BBCA)
Project Start
1985-07-01
Project End
2000-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
11
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Princeton University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544

  Publications

Zhang, H; Lizitsa, N; Bryant, R G et al. (2001) Experimental characterization of intermolecular multiple-quantum coherence pumping efficiency in solution NMR. J Magn Reson 148:200-8
Lin, Y Y; Lisitza, N; Ahn, S et al. (2000) Resurrection of crushed magnetization and chaotic dynamics in solution NMR spectroscopy. Science 290:118-22
Rizi, R R; Ahn, S; Alsop, D C et al. (2000) Intermolecular zero-quantum coherence imaging of the human brain. Magn Reson Med 43:627-32
Richter, W; Richter, M; Warren, W S et al. (2000) Functional magnetic resonance imaging with intermolecular multiple-quantum coherences. Magn Reson Imaging 18:489-94
Garrett-Roe, S; Warren, W S (2000) Numerical studies of intermolecular multiple quantum coherences: high-resolution NMR in inhomogeneous fields and contrast enhancement in MRI. J Magn Reson 146:13-Jan
Lin, Y; Ahn, S; Murali, N et al. (2000) High-resolution, >1 GHz NMR in unstable magnetic fields. Phys Rev Lett 85:3732-5
Warren, W S; Ahn, S; Mescher, M et al. (1998) MR imaging contrast enhancement based on intermolecular zero quantum coherences. Science 281:247-51
Ahn, S; Warren, W S; Lee, S (1997) Quantum treatment of intermolecular multiple-quantum coherences with intramolecular J coupling in solution NMR. J Magn Reson 128:114-29
Vathyam, S; Lee, S; Warren, W S (1996) Homogeneous NMR spectra in inhomogeneous fields. Science 272:92-6
Richter, W; Lee, S; Warren, W S et al. (1995) Imaging with intermolecular multiple-quantum coherences in solution nuclear magnetic resonance. Science 267:654-7

Showing the most recent 10 out of 12 publications