Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035332-10
Application #
2177863
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1986-12-01
Project End
1998-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Hernandez, Jose A; Phillips, Aaron H; Erbil, W Kaya et al. (2011) A sterile alpha-motif domain in NafY targets apo-NifDK for iron-molybdenum cofactor delivery via a tethered domain. J Biol Chem 286:6321-8
Soboh, Basem; Boyd, Eric S; Zhao, Dehua et al. (2010) Substrate specificity and evolutionary implications of a NifDK enzyme carrying NifB-co at its active site. FEBS Lett 584:1487-92
Hernandez, Jose A; Curatti, Leonardo; Aznar, Constantino P et al. (2008) Metal trafficking for nitrogen fixation: NifQ donates molybdenum to NifEN/NifH for the biosynthesis of the nitrogenase FeMo-cofactor. Proc Natl Acad Sci U S A 105:11679-84
George, Simon J; Igarashi, Robert Y; Piamonteze, Cinthia et al. (2007) Identification of a Mo-Fe-S cluster on NifEN by Mo K-edge extended X-ray absorption fine structure. J Am Chem Soc 129:3060-1
Curatti, Leonardo; Hernandez, Jose A; Igarashi, Robert Y et al. (2007) In vitro synthesis of the iron-molybdenum cofactor of nitrogenase from iron, sulfur, molybdenum, and homocitrate using purified proteins. Proc Natl Acad Sci U S A 104:17626-31
Hernandez, Jose A; Igarashi, Robert Y; Soboh, Basem et al. (2007) NifX and NifEN exchange NifB cofactor and the VK-cluster, a newly isolated intermediate of the iron-molybdenum cofactor biosynthetic pathway. Mol Microbiol 63:177-92
Soboh, Basem; Igarashi, Robert Y; Hernandez, Jose A et al. (2006) Purification of a NifEN protein complex that contains bound molybdenum and a FeMo-Co precursor from an Azotobacter vinelandii DeltanifHDK strain. J Biol Chem 281:36701-9
Dyer, David H; Rubio, Luis M; Thoden, James B et al. (2003) The three-dimensional structure of the core domain of Naf Y from Azotobacter vinelandii determined at 1.8-A resolution. J Biol Chem 278:32150-6
Ruttimann-Johnson, Carmen; Rubio, Luis M; Dean, Dennis R et al. (2003) VnfY is required for full activity of the vanadium-containing dinitrogenase in Azotobacter vinelandii. J Bacteriol 185:2383-6
Rubio, Luis M; Rangaraj, Priya; Homer, Mary J et al. (2002) Cloning and mutational analysis of the gamma gene from Azotobacter vinelandii defines a new family of proteins capable of metallocluster binding and protein stabilization. J Biol Chem 277:14299-305

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