This work involves general characterization of enzymes involved in respiration. The subject organism is the thermophilic bacterium Thermus thermophilus. With a working knowledge of its biology, biochemistry and molecular biology, it has been possible to carry out a wide range of biochemical, biophysical, and physical characterizations of its respiratory proteins. Because the respiratory proteins from this organism are functional and structural homologs of their eukaryotic counterparts, the results of our structure-function studies are directly applicable to all other such enzymes, notably those of the mammalian mitochondrion. During the past funding period we: (1) Determined the DNA sequence of structural genes encoding cytochromes caa3 and ba3, and by making detailed comparisons of their deduced amino acid sequences demonstrated that they are members of heme/Cu oxidase super-family. (2) We carried out a number of spectroscopic investigations with cytochromes caa3 and ba3 and of Rieske-type proteins, which led to detailed new information about the active site structures of these proteins. (3) Our most exciting discovery was that a small portion of the subunit II of cytochrome ba3 gene could be subcloned, expressed in E. coli, and was found to form a very stable CuA center. This discovery opens many new research opportunites for exploring the properties of this novel and somewhat unexpected arrangement of Cu atoms, and this is a very fast moving field of research. Accordingly, the current proposal is tightly focussed on just two scientific goals while the longer-term goals of the project are served under a much broader technical goal.
The Specific Aims are: (1) Toward achieving a detailed understanding of CuA structure and function, we will assemble and interpret spectral, structural and functional information from a water-soluble, CuA containing protein. (2) As a first step toward understanding assembly of the CuA center, we will examine the properties of a yellow-colored intermediate that forms during CuA synthesis. (3) We will continue our efforts to develop a combined genetic, structural and kinetic 'system' that will permit detailed biophysical study of the cytochrome ba3. The significance of this work to human health lies in a deeper understanding of the enzymes that provide energy to all cells. That mutations in human respiratory proteins are being found at a prodigious rate, is evidenced by the appearance of a new web site, http://infinity.gen.emory.edu/mitomap.html.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM035342-14
Application #
2022058
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1984-12-01
Project End
2000-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
14
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
McDonald, William; Funatogawa, Chie; Li, Yang et al. (2014) Conserved glycine 232 in the ligand channel of ba3 cytochrome oxidase from Thermus thermophilus. Biochemistry 53:4467-75
McDonald, William; Funatogawa, Chie; Li, Yang et al. (2013) Ligand access to the active site in Thermus thermophilus ba(3) and bovine heart aa(3) cytochrome oxidases. Biochemistry 52:640-52
Luna, V Mitch; Fee, James A; Deniz, Ashok A et al. (2012) Mobility of Xe atoms within the oxygen diffusion channel of cytochrome ba(3) oxidase. Biochemistry 51:4669-76
Neehaul, Yashvin; Chen, Ying; Werner, Carolin et al. (2012) Electrochemical and infrared spectroscopic analysis of the interaction of the Cu(A) domain and cytochrome c(552) from Thermus thermophilus. Biochim Biophys Acta 1817:1950-4
Chang, Hsin-Yang; Choi, Sylvia K; Vakkasoglu, Ahmet Selim et al. (2012) Exploring the proton pump and exit pathway for pumped protons in cytochrome ba3 from Thermus thermophilus. Proc Natl Acad Sci U S A 109:5259-64
Egawa, Tsuyoshi; Chen, Ying; Fee, James A et al. (2012) The rate-limiting step in O(2) reduction by cytochrome ba(3) from Thermus thermophilus. Biochim Biophys Acta 1817:666-71
Liu, Bin; Zhang, Yang; Sage, J Timothy et al. (2012) Structural changes that occur upon photolysis of the Fe(II)(a3)-CO complex in the cytochrome ba(3)-oxidase of Thermus thermophilus: a combined X-ray crystallographic and infrared spectral study demonstrates CO binding to Cu(B). Biochim Biophys Acta 1817:658-65
Tiefenbrunn, Theresa; Liu, Wei; Chen, Ying et al. (2011) High resolution structure of the ba3 cytochrome c oxidase from Thermus thermophilus in a lipidic environment. PLoS One 6:e22348
Farver, Ole; Wherland, Scot; Antholine, William E et al. (2010) Pulse Radiolysis Studies of Temperature Dependent Electron Transfers among Redox Centers in ba(3)-Cytochrome c Oxidase from Thermus thermophilus: Comparison of A- and B-Type Enzymes. Biochemistry :
Szundi, Istvan; Funatogawa, Chie; Fee, James A et al. (2010) CO impedes superfast O2 binding in ba3 cytochrome oxidase from Thermus thermophilus. Proc Natl Acad Sci U S A 107:21010-5

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