Abstract

The overall objective is to elucidate some of the mechanisms for the control and regulation of DNA-dependent RNA transcription in chloroplasts of Euglena gracilis. Specific projects include the following: (1) Location and DNA sequence analysis of chloroplast transcription units and putative regulatory regions, (2) Quantitation of changes in specific transcripts during light induced chloroplast development, (3) Characterizing in an in vitro system the components involved in selective RNA transcription. In the coming year we plan the following projects: (1) Continue DNA sequence analysis of portions of the ribosomal RNA transcription units, and selected mRNA coding regions, including the gene for the large subunit of ribulose-1,5 -bis phosphate carboxylase, (2) Complete a study of the subunit structure of chloroplast RNA polymerase, (3) Analyze the primary transcripts and processing pathways via solution and Northern hybridization analysis for some specific transcription units for which DNA sequence data are available, (4) Continue a general program of mapping, cloning, and gene localization for the Euglena chloroplast genome.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035665-03
Application #
3288662
Study Section
Molecular Biology Study Section (MBY)
Project Start
1985-02-01
Project End
1988-04-02
Budget Start
1986-07-01
Budget End
1988-04-02
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Arizona
Department
Type
Schools of Arts and Sciences
DUNS #
City
Tucson
State
AZ
Country
United States
Zip Code
85722

  Publications

Sheveleva, Elena V; Hallick, Richard B (2004) Recent horizontal intron transfer to a chloroplast genome. Nucleic Acids Res 32:803-10
Sheveleva, Elena V; Giordani, Nicole V; Hallick, Richard B (2002) Identification and comparative analysis of the chloroplast alpha-subunit gene of DNA-dependent RNA polymerase from seven Euglena species. Nucleic Acids Res 30:1247-54
Doetsch, N A; Thompson, M D; Favreau, M R et al. (2001) Comparison of psbK operon organization and group III intron content in chloroplast genomes of 12 Euglenoid species. Mol Gen Genet 264:682-90
Doetsch, N A; Favreau, M R; Kuscuoglu, N et al. (2001) Chloroplast transformation in Euglena gracilis: splicing of a group III twintron transcribed from a transgenic psbK operon. Curr Genet 39:49-60
Doetsch, N A; Thompson, M D; Hallick, R B (1998) A maturase-encoding group III twintron is conserved in deeply rooted euglenoid species: are group III introns the chicken or the egg? Mol Biol Evol 15:76-86
Stevenson, J K; Hallick, R B (1994) The psaA operon pre-mRNA of the Euglena gracilis chloroplast is processed into photosystem I and II mRNAs that accumulate differentially depending on the conditions of cell growth. Plant J 5:247-60
Hong, L; Hallick, R B (1994) Gene structure and expression of a novel Euglena gracilis chloroplast operon encoding cytochrome b6 and the beta and epsilon subunits of the H(+)-ATP synthase complex. Curr Genet 25:270-81
Drager, R G; Hallick, R B (1993) A novel Euglena gracilis chloroplast operon encoding four ATP synthase subunits and two ribosomal proteins contains 17 introns. Curr Genet 23:271-80
Drager, R G; Hallick, R B (1993) A complex twintron is excised as four individual introns. Nucleic Acids Res 21:2389-94
Copertino, D W; Shigeoka, S; Hallick, R B (1992) Chloroplast group III twintron excision utilizing multiple 5'- and 3'-splice sites. EMBO J 11:5041-50

Showing the most recent 10 out of 16 publications