The DNA polymerase III holoenzyme of E. coli is a prototypical replicative complex, exhibiting properties in common with other cellular replicases, including a high rate of processive elongation and the ability to interact with other proteins at the replication fork, establishing the communication channels necessary to coordinate the events required for efficient chromosomal replication. A key component of all cellular replicases is a multi-subunit assembly of homologous proteins that require ATP to assemble a 'sliding clamp processivity factor' onto primer termini. In E.coli, this function is served by the DnaX complex, DnaX3-delta-delta-chi-psi. The dnaX gene of E.coli encodes two distinct products: tau, the full-length translation product and gamma, a shorter protein that arises by translational frameshifting. In spite of gamma and tau being found together within holoenzyme, they do not readily form mixed complexes in vitro or when overexpressed in vivo. During the last grant period, we have determined the limits of five domains of the tau DnaX protein, the three domains of the homologous delta-prime subunit, and have defined the subunit interactions of each domain. During the next grant period, we will determine the factors required for proper DnaX complex assembly using an in vitro assay we have recently developed. We will refine our understanding of the subunit interface domains down to the amino acid level. Lastly, we have developed a system that permits individually assessing the occupancy of each polymerase site of the dimeric holoenzyme with primer-templates. This system will be used to determine whether the internal DnaX clamp loader can assemble beta2 for both strands of DNA at the replication fork, and to further investigate our asymmetric dimer hypothesis. These studies are expected to significantly improve our understanding of the structure and function of this important and highly conserved enzyme system, to provide insight into how living systems assemble their multisubunit complexes that load processivity factors onto DNA, and to help us understand the functional advantages of such complexes.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM035695-14A1
Application #
6256425
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Program Officer
Wolfe, Paul B
Project Start
1985-12-01
Project End
2004-08-31
Budget Start
2000-09-15
Budget End
2001-08-31
Support Year
14
Fiscal Year
2000
Total Cost
$271,800
Indirect Cost
Name
University of Colorado Denver
Department
Biochemistry
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045
Downey, Christopher D; McHenry, Charles S (2010) Chaperoning of a replicative polymerase onto a newly assembled DNA-bound sliding clamp by the clamp loader. Mol Cell 37:481-91
Yuan, Quan; McHenry, Charles S (2009) Strand displacement by DNA polymerase III occurs through a tau-psi-chi link to single-stranded DNA-binding protein coating the lagging strand template. J Biol Chem 284:31672-9
Jarvis, Thale C; Beaudry, Amber A; Bullard, James M et al. (2005) Discovery and characterization of the cryptic psi subunit of the pseudomonad DNA replicase. J Biol Chem 280:40465-73
Bullard, James M; Pritchard, Arthur E; Song, Min-Sun et al. (2002) A three-domain structure for the delta subunit of the DNA polymerase III holoenzyme delta domain III binds delta' and assembles into the DnaX complex. J Biol Chem 277:13246-56
Song, M S; McHenry, C S (2001) Carboxyl-terminal domain III of the delta' subunit of DNA polymerase III holoenzyme binds DnaX and supports cooperative DnaX complex assembly. J Biol Chem 276:48709-15
Glover, B P; Pritchard, A E; McHenry, C S (2001) tau binds and organizes Escherichia coli replication proteins through distinct domains: domain III, shared by gamma and tau, oligomerizes DnaX. J Biol Chem 276:35842-6
Song, M S; Dallmann, H G; McHenry, C S (2001) Carboxyl-terminal domain III of the delta' subunit of the DNA polymerase III holoenzyme binds delta. J Biol Chem 276:40668-79
Gao, D; McHenry, C S (2001) Tau binds and organizes Escherichia coli replication proteins through distinct domains. Domain III, shared by gamma and tau, binds delta delta ' and chi psi. J Biol Chem 276:4447-53
Gao, D; McHenry, C S (2001) tau binds and organizes Escherichia coli replication through distinct domains. Partial proteolysis of terminally tagged tau to determine candidate domains and to assign domain V as the alpha binding domain. J Biol Chem 276:4433-40
Glover, B P; McHenry, C S (2001) The DNA polymerase III holoenzyme: an asymmetric dimeric replicative complex with leading and lagging strand polymerases. Cell 105:925-34

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