We have created and implemented a microwave-powered chemical reaction interface which is attached to a mass spectrometer as a detection device. This interface atomizes molecules which enter it and, through the addition of a reactant gas, it converts the elements initially present into stable volatile polyatomic species whose mass spectra can be used to quantify those elements using conventional mass spectrometry. This approach appears applicable to a variety of samples including drugs and macromolecules and sampling techniques such as gas chromatography, high pressure liquid chromatography or direct introduction. Many elements, such as carbon, nitrogen, sulfur, halogens, or hydrogen, or specific nuclides, such as 14C or 15N can be monitored in a selective and sensitive manner using mass spectrometry. The goals of this project are to establish the pharmacological capabilities of this instrumental concept, and to develop the necessary hardware to accomodate a variety of input devices. The potential of the reaction interface/mass spectrometer concept suggests a wider range of applicability as a selective detector to both pharmacological studies and to chemical analysis in general than any other device presently available.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036143-03
Application #
3289710
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1986-01-01
Project End
1989-12-31
Budget Start
1988-01-01
Budget End
1988-12-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
George Washington University
Department
Type
Schools of Medicine
DUNS #
City
Washington
State
DC
Country
United States
Zip Code
20052
Abramson, F P (2001) The use of stable isotopes in drug metabolism studies. Semin Perinatol 25:133-8
Abramson, F P; Black, G E; Lecchi, P (2001) Application of high-performance liquid chromatography with isotope-ratio mass spectrometry for measuring low levels of enrichment of underivatized materials. J Chromatogr A 913:269-73
Eckers, C; Abramson, F P; Lecchi, P (2001) Detection of sulfur-containing impurities in pharmaceutical samples by high performance liquid chromatography/chemical reaction interface mass spectrometry. Rapid Commun Mass Spectrom 15:602-7
Lecchi, P; Abramson, F P (2000) An innovative method for measuring hydrogen and deuterium: chemical reaction interface mass spectrometry with nitrogen reactant gas. J Am Soc Mass Spectrom 11:400-6
Lecchi, P; Abramson, F P (1999) Size exclusion chromatography-chemical reaction interface mass spectrometry: ""a perfect match"". Anal Chem 71:2951-5
Chen, P; Teffera, Y; Black, G E et al. (1999) Flow injection with chemical reaction interface-isotope ratio mass spectrometry: an alternative to off-line combustion for detecting low levels of enriched 13C in mass balance studies. J Am Soc Mass Spectrom 10:153-8
Abramson, F P (1999) A mass spectrum that today is rarely seen. J Am Soc Mass Spectrom 10:76-7
Teng, J; Teffera, Y; McLean, M et al. (1998) Studying the reaction between clozapine and glutathione with element-selective detection. Res Commun Mol Pathol Pharmacol 99:131-42
Chen, P; Abramson, F P (1998) Measuring DNA synthesis rates with [1-13C]glycine. Anal Chem 70:1664-9
Osborn, B L; Abramson, F P (1998) Pharmacokinetic and metabolism studies using uniformly stable isotope labeled proteins with HPLC/CRIMS detection. Biopharm Drug Dispos 19:439-44

Showing the most recent 10 out of 16 publications