We propose to analyze two problems by the methods of elasticity theory. The first problem is to find the path of a thin elastic rod (DNA) attracted in toward a ring (histone octamer surface). In this way we hope to explain neutron diffraction data which indicate that nucleosomal DNA undergoes an upwinding transition at low ionic strength. The second problem is to find the path of a thin elastic rod (DNA) constrained to lie on a cylinder (histone octamer surface). We hope the solution to this problem will help us understand the observed trajectory of nucleosomal DNA. We propose also to study a problem in polyelectrolyte theory, the field-induced aggregation of polyions, in order to suggest a plausible structure for DNA aggregates recently observed to form under the action of an applied electric field. This problem bears on the interpretation of electro-optical data on chromatin.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
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Biophysics and Biophysical Chemistry A Study Section (BBCA)
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Rutgers University
Schools of Arts and Sciences
New Brunswick
United States
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Kosikov, Konstantin M; Gorin, Andrey A; Lu, Xiang-Jun et al. (2002) Bending of DNA by asymmetric charge neutralization: all-atom energy simulations. J Am Chem Soc 124:4838-47
Manning, Gerald S (2002) Electrostatic free energy of the DNA double helix in counterion condensation theory. Biophys Chem 101-102:461-73
Volker, J; Klump, H H; Manning, G S et al. (2001) Counterion association with native and denatured nucleic acids: an experimental approach. J Mol Biol 310:1011-25
Manning, G S; Ray, J (1998) Counterion condensation revisited. J Biomol Struct Dyn 16:461-76
Fenley, M O; Manning, G S; Marky, N L et al. (1998) Excess counterion binding and ionic stability of kinked and branched DNA. Biophys Chem 74:135-52
Manning, G S (1995) An elastic model for conformational transitions of spacer DNA in chromatin;first results. J Biomol Struct Dyn 12:1083-101
Fenley, M O; Olson, W K; Tobias, I et al. (1994) Electrostatic effects in short superhelical DNA. Biophys Chem 50:255-71
Manning, G S (1993) An elastic model for in-plane deformations of small DNA rings. J Biomol Struct Dyn 10:657-73
Ray, J; Manning, G S (1992) Theory of delocalized ionic binding to polynucleotides: structural and excluded-volume effects. Biopolymers 32:541-9
Marky, N L; Manning, G S (1991) The elastic resilience of DNA can induce all-or-none structural transitions in the nucleosome core particle. Biopolymers 31:1543-57

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