Abstract

Proposed an external point-charge model to explain the variance in absorption maxima of visual pigments, an issue of prime importance because the variance has a direct bearing to therecognition of color vision, an area in which our knowledge is extremely limited. Our model was proposed on the basis of the maxima of numerous artificial bovine rhodopsins which were prepared from synthetic retinals have specific structures. We will incorporate these various artificial retinals with opsins from sources other than bovine, such as octopus and chicken. A table of absorption maxima and thus give us a clearer picture of the external point-charge model (we have recently proposed a model to rationalize the purple color of purple membrane, the chromophore of which is again all-trans retinal). Emphasis will also be placed on peptide mapping within the binding site via photo-affinity labelling, and in making artificial rhodopsins which could yield structural information on the bleaching intermediates. Efforts will also continue to be directed towards the synthesis of chormophores which theoretically could shift the vision into regions beyond the normal range.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036564-15
Application #
3290795
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1979-03-01
Project End
1991-02-28
Budget Start
1988-03-01
Budget End
1989-02-28
Support Year
15
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Type
Graduate Schools
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027

  Publications

Foster, Kenneth W; Saranak, Jureepan; Krane, Sonja et al. (2011) Evidence from Chlamydomonas on the photoactivation of rhodopsins without isomerization of their chromophore. Chem Biol 18:733-42
Brown, Michael F; Martínez-Mayorga, Karina; Nakanishi, Koji et al. (2009) Retinal conformation and dynamics in activation of rhodopsin illuminated by solid-state H NMR spectroscopy. Photochem Photobiol 85:442-53
Pescitelli, Gennaro; Sreerama, Narasimha; Salvadori, Piero et al. (2008) Inherent chirality dominates the visible/near-ultraviolet CD spectrum of rhodopsin. J Am Chem Soc 130:6170-81
Chen, Yihui; Herrmann, Rolf; Fishkin, Nathan et al. (2008) Synthesis and spectroscopic characterization of photo-affinity peptide ligands to study rhodopsin-G protein interaction. Photochem Photobiol 84:831-8
Chen, Yihui; Parr, Timothy; Holmes, Andrea E et al. (2008) Porphyrinmaleimides: towards thiol probes for cysteine residues in proteins. Bioconjug Chem 19:5-9
Kim, So R; He, Jiangtao; Yanase, Emiko et al. (2007) Characterization of dihydro-A2PE: an intermediate in the A2E biosynthetic pathway. Biochemistry 46:10122-9
Brown, Michael F; Heyn, Maarten P; Job, Constantin et al. (2007) Solid-state 2H NMR spectroscopy of retinal proteins in aligned membranes. Biochim Biophys Acta 1768:2979-3000
Abeywickrama, Chandima; Matsuda, Hiroko; Jockusch, Steffen et al. (2007) Immunochemical recognition of A2E, a pigment in the lipofuscin of retinal pigment epithelial cells. Proc Natl Acad Sci U S A 104:14610-5
Struts, Andrey V; Salgado, Gilmar F J; Tanaka, Katsunori et al. (2007) Structural analysis and dynamics of retinal chromophore in dark and meta I states of rhodopsin from 2H NMR of aligned membranes. J Mol Biol 372:50-66
Washington, Ilyas; Zhou, Jilin; Jockusch, Steffen et al. (2007) Chlorophyll derivatives as visual pigments for super vision in the red. Photochem Photobiol Sci 6:775-9

Showing the most recent 10 out of 60 publications